Comparison of a simplified whole blood and isolated lymphocyte stimulation technique.
Comparison of a simplified whole blood and isolated lymphocyte stimulation technique.
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简化全血和分离淋巴细胞刺激技术的比较。
DOI：
10.3109/08820138109050707
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
M. Stein
中科院分区：
文献类型：
--
作者：
S. Keller;S. Schleifer;J. Sherman;M. Camerino;H. Smith;M. Stein
利用PHA、ConA和PWM对健康男性的全血和分离淋巴细胞刺激进行比较。两种淋巴细胞刺激方法对3种有丝分裂原的剂量反应曲线不同。随着培养时间的延长，脉宽调制反应在全血和分离淋巴细胞试验中均显著增加。在全血法中，增加培养时间对PHA培养没有影响，但增加了对ConA的反应。在每项研究中，3h -胸腺嘧啶的反应与125IUdR的反应相当。
Whole blood and isolated lymphocyte stimulation in healthy men were compared utilizing PHA, ConA, and PWM. Dose response curves with each of the 3 mitogens were found to differ with the two techniques of lymphocytes stimulation. When incubation time was lengthened PWM responses increased markedly in both the whole blood and isolated lymphocyte assays. Increased incubation time had no effect on PHA cultures and increased the responses with ConA in the whole blood method. In each study, responses with 3H-thymidine were comparable to responses utilizing 125IUdR.