RIBOSOMAL-RNA-MESSENGER-RNA BASE-PAIRING STIMULATES A PROGRAMMED -1-RIBOSOMAL FRAMESHIFT
RIBOSOMAL-RNA-MESSENGER-RNA BASE-PAIRING STIMULATES A PROGRAMMED -1-RIBOSOMAL FRAMESHIFT
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DOI：
10.1128/jb.176.22.6842-6851.1994
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发表时间：
1994-11-01
影响因子：
3.2
通讯作者：
ATKINS, JF
中科院分区：
文献类型：
--
作者：
LARSEN, B;WILLS, NM;ATKINS, JF
16 SrRNA 3'端与mRNA的碱基配对对大肠杆菌dnaX基因的程序性移码很重要。这种配对与原核生物核糖体在选择翻译起始子时使用的Shine-Dalgarno配对相同，但对于移码，相互作用发生在延长核糖体内。对于dnaX-1移码，Shine-Dalgarno序列的3'碱基是移位位点5'的10个核苷酸。此前，Shine-Dalgarno rRNA-mRNA配对被证明可以刺激解码释放因子2基因所需的+1移码。然而，在释放因子2基因中，Shine-Dalgarno序列位于移位位点5'的3个核苷酸处。当Shine-Dalgarno序列移动到相对于dnaX移位位点的相同位置时，它是抑制性的而不是刺激性的。通过延长核糖体的Shine-Dalgarno相互作用可能用于刺激多种基因解码中的移码。
Base pairing between the 3' end of 16S rRNA and mRNA is shown to be important for the programmed -1 frameshifting utilized in decoding the Escherichia coli dnaX gene. This pairing is the same as the Shine-Dalgarno pairing used by prokaryotic ribosomes in selection of translation initiators, but for frameshifting the interaction occurs within elongating ribosomes. For dnaX -1 frameshifting, the 3' base of the Shine-Dalgarno sequence is 10 nucleotides 5' of the shift site. Previously, Shine-Dalgarno rRNA-mRNA pairing was shown to stimulate the +1 frameshifting necessary for decoding the release factor 2 gene. However, in the release factor 2 gene, the Shine-Dalgarno sequence is located 3 nucleotides 5' of the shift site. When the Shine-Dalgarno sequence is moved to the same position relative to the dnaX shift site, it is inhibitory rather than stimulatory. Shine-Dalgarno interactions by elongating ribosomes are likely to be used in stimulating -1 frameshifting in the decoding of a variety of genes.