Temporal Organization of the Sleep-Wake Cycle under Food Entrainment in the Rat
Temporal Organization of the Sleep-Wake Cycle under Food Entrainment in the Rat
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DOI：
10.5665/sleep.5982
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发表时间：
2016-07-01
期刊：
影响因子：
5.6
通讯作者：
Ocampo-Garces, Adrian
中科院分区：
文献类型：
--
作者：
Castro-Faundez, Javiera;Diaz, Javier;Ocampo-Garces, Adrian
研究目的：分析大鼠在食物夹带条件下睡眠-觉醒周期的时间组织。方法：18只雄性SD大鼠慢性植入进行多导睡眠图记录。在基线(BL)方案中，大鼠按照12：12明暗(LD)时间表在单独的隔离室中自由地记录食物和水。食物夹带是通过4h食物限制(FR)方案进行的，从光时间5开始。8只动物经历了FR方案的3h推进(A-FR)。我们比较了在光照和食物夹带下以及在预定食物递送阶段提前一段时间后，觉醒、非快速眼动睡眠和快速眼动睡眠的平均曲线和峰期。我们进一步评估了REM睡眠动态平衡和NREM睡眠脑电Delta波谱。结果：在FR持续9天或更长时间后，观察到显著的食物预期唤醒间期，其特征是觉醒增加，REM睡眠倾向受到抑制，NREM睡眠EEG Delta活动减弱。在FR下，REM睡眠表现出强烈的夜间相位偏好，这不能用夜间REM睡眠反弹来解释。在A-FR方案中，睡眠-觉醒状态和NREM睡眠脑电增量活动的平均曲线与进餐时间保持相位锁定。结论：我们的结果支持这样的假设，即在食物夹带下，睡眠-觉醒周期耦合到食物夹带振荡器(FEO)。我们的发现表明，FEO输出和NREM睡眠EEG增量活动发生器之间存在意想不到的相互作用。
Study Objectives: To analyze the temporal organization of the sleep-wake cycle under food entrainment in the rat.Methods: Eighteen male Sprague-Dawley rats were chronically implanted for polysomnographic recording. During the baseline (BL) protocol, rats were recorded under a 12: 12 light-dark (LD) schedule in individual isolation chambers with food and water ad libitum. Food entrainment was performed by means of a 4-h food restriction (FR) protocol starting at photic zeitgeber time 5. Eight animals underwent a 3-h phase advance of the FR protocol (A-FR). We compared the mean curves and acrophases of wakefulness, NREM sleep, and REM sleep under photic and food entrainment and after a phase advance in scheduled food delivery. We further evaluated the dynamics of REM sleep homeostasis and the NREM sleep EEG delta wave profile.Results: A prominent food-anticipatory arousal interval was observed after nine or more days of FR, characterized by increased wakefulness and suppression of REM sleep propensity and dampening of NREM sleep EEG delta activity. REM sleep exhibited a robust nocturnal phase preference under FR that was not explained by a nocturnal REM sleep rebound. The mean curve of sleep-wake states and NREM sleep EEG delta activity remained phase-locked to the timing of meals during the A-FR protocol.Conclusions: Our results support the hypothesis that under food entrainment, the sleep-wake cycle is coupled to a food-entrainable oscillator (FEO). Our findings suggest an unexpected interaction between FEO output and NREM sleep EEG delta activity generators.