Favourable influence of hydrophobic surfaces on protein structure in porous organically-modified silica glasses.
Favourable influence of hydrophobic surfaces on protein structure in porous organically-modified silica glasses.
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DOI：
10.1016/j.biomaterials.2008.02.026
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发表时间：
2008-06
期刊：
影响因子：
14
通讯作者：
B. Menaa;M. Herrero;V. Rives;M. Lavrenko;D. Eggers
中科院分区：
文献类型：
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作者：
B. Menaa;M. Herrero;V. Rives;M. Lavrenko;D. Eggers
使用有机改性硅氧烷作为主体材料来研究拥挤环境中表面化学对蛋白质构象的影响。溶胶-凝胶材料由四甲氧基硅烷和一系列单取代烷氧基硅烷RSi(OR')3制备，其特征是R位上链长不断增加的烷基。使用远紫外区域的圆二色光谱，发现随着疏水性前体的含量从 0% 增加到 15%，脱辅基肌红蛋白从未折叠状态转变为类似天然的螺旋状态。在RSi(OR')3摩尔含量固定为5%时，脱辅基肌红蛋白的螺旋结构随着R基团链长的增加而增加，即甲基<乙基<正丙基<正丁基<正己基。核糖核酸酶 A 的三级结构也观察到了这种趋势，表明蛋白质折叠和生物活性对邻近表面的亲水/疏水平衡敏感。观察到的蛋白质结构变化与改性玻璃的总表面积或平均孔径无关，但扫描电子显微镜图像揭示了表面形态和烷基链长度之间的有趣关系。将低含量的疏水基团掺入亲水表面的意想不到的好处可能会导致材料具有改善的生物相容性，用于生物传感器和植入装置。
Organically-modified siloxanes were used as host materials to examine the influence of surface chemistry on protein conformation in a crowded environment. The sol–gel materials were prepared from tetramethoxysilane and a series of monosubstituted alkoxysilanes, RSi(OR′)3, featuring alkyl groups of increasing chain length in the R-position. Using circular dichroism spectroscopy in the far-UV region, apomyoglobin was found to transit from an unfolded state to a native-like helical state as the content of the hydrophobic precursor increased from 0 to 15%. At a fixed molar content of 5% RSi(OR′)3, the helical structure of apomyoglobin increased with the chain length of the R-group, i.e. methyl<ethyl<n-propyl<n-butyl<n-hexyl. This trend also was observed for the tertiary structure of ribonuclease A, suggesting that protein folding and biological activity are sensitive to the hydrophilic/hydrophobic balance of neighboring surfaces. The observed changes in protein structure did not correlate with total surface area or the average pore size of the modified glasses, but scanning electron microscopy images revealed an interesting relationship between surface morphology and alkyl chain length. The unexpected benefit of incorporating a low content of hydrophobic groups into a hydrophilic surface may lead to materials with improved biocompatibility for use in biosensors and implanted devices.