Listeria monocytogenes Differential Transcriptome Analysis Reveals Temperature-Dependent Agr Regulation and Suggests Overlaps with Other Regulons
Listeria monocytogenes Differential Transcriptome Analysis Reveals Temperature-Dependent Agr Regulation and Suggests Overlaps with Other Regulons
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DOI：
10.1371/journal.pone.0043154
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发表时间：
2012-09-14
期刊：
影响因子：
3.7
通讯作者：
Piveteau, Pascal
中科院分区：
文献类型：
--
作者：
Garmyn, Dominique;Augagneur, Yoann;Piveteau, Pascal
单核细胞增生李斯特菌是一种普遍存在的机会致病微生物。环境适应需要基因表达的持续调节。在转录调节因子中，AgrA是自诱导系统的一部分。温度是一种对体内适应至关重要的环境线索。为了研究温度如何影响AgrA依赖的转录，我们比较了亲本菌株L.单核细胞增生EGD-e及其Δ agrA突变体在25 ℃的恒温温度和37 ℃的体内温度下的生长曲线。转录组的变化在37摄氏度比在25摄氏度高。结果表明，AgrA可能参与氮转运、氨基酸、嘌呤和嘧啶生物合成途径以及噬菌体相关功能的调节。放松管制导致37摄氏度下的生长优势，但影响耐盐性。最后，我们的研究结果表明与PrfA，sigma B，sigma H和CodY调节子重叠。这些重叠可能表明，通过AgrA，单核细胞增生李斯特菌整合其生物环境的信息。
Listeria monocytogenes is a ubiquitous, opportunistic pathogenic organism. Environmental adaptation requires constant regulation of gene expression. Among transcriptional regulators, AgrA is part of an auto-induction system. Temperature is an environmental cue critical for in vivo adaptation. In order to investigate how temperature may affect AgrA-dependent transcription, we compared the transcriptomes of the parental strain L. monocytogenes EGD-e and its Delta agrA mutant at the saprophytic temperature of 25 degrees C and in vivo temperature of 37 degrees C. Variations of transcriptome were higher at 37 degrees C than at 25 degrees C. Results suggested that AgrA may be involved in the regulation of nitrogen transport, amino acids, purine and pyrimidine biosynthetic pathways and phage-related functions. Deregulations resulted in a growth advantage at 37 degrees C, but affected salt tolerance. Finally, our results suggest overlaps with PrfA, sigma B, sigma H and CodY regulons. These overlaps may suggest that through AgrA, Listeria monocytogenes integrates information on its biotic environment.