MODELS OF HUMAN METASTATIC COLON CANCER IN NUDE-MICE ORTHOTOPICALLY CONSTRUCTED BY USING HISTOLOGICALLY INTACT PATIENT SPECIMENS
MODELS OF HUMAN METASTATIC COLON CANCER IN NUDE-MICE ORTHOTOPICALLY CONSTRUCTED BY USING HISTOLOGICALLY INTACT PATIENT SPECIMENS
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DOI：
10.1073/pnas.88.20.9345
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发表时间：
1991-10-01
影响因子：
11.1
通讯作者：
HOFFMAN, RM
中科院分区：
文献类型：
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作者：
FU, XY;BESTERMAN, JM;HOFFMAN, RM
迫切需要与临床相关的人类癌症动物模型。为了实现这一目标，从手术中获得的组织学完整的人类结肠癌标本被原位植入裸鼠的结肠或盲肠。我们在20例植入性结肠肿瘤患者中观察到13例广泛的原位生长。这些肿瘤表现出不同的生长模式，随后出现局部、淋巴结和肝脏转移，以及一般性腹部癌。因此，已经开发的人类结肠癌模型显示(i)局部生长，（ii）腹部转移，（iii）伴有广泛腹膜播种的一般性腹部癌病，（iv）淋巴结转移，(v)肝转移，（vi）结肠梗阻。这些模型允许肿瘤通过形成大的队列。他们将促进结肠癌转移能力的生物学研究和开发抗转移性癌症的新药。这些模型还可以预测个体患者癌症的临床病程和体内对药物的反应。
There is an important need for clinically relevant animal models for human cancers. Toward this goal, histologically intact human colon-cancer specimens derived surgically from patients were implanted orthotopically to the colon or cecum of nude mice. We have observed extensive orthotopic growth in 13 of 20 cases of implanted patient colon tumors. These showed various growth patterns with subsequent regional, lymph-node, and liver metastasis, as well as general abdominal carcinomatosis. Thus, models for human colon cancer have been developed that show (i) local growth, (ii) abdominal metastasis, (iii) general abdominal carcinomatosis with extensive peritoneal seeding, (iv) lymph-node metastasis, (v) liver metastasis, and (vi) colonic obstruction. These models permit the passage of the tumors to form large cohorts. They will facilitate research into the biology of colon cancer metastatic capability and the development of new drugs active against metastatic cancer. These models may also predict the clinical course and the in vivo response to drugs of the cancer of individual patients.