Human neutrophils express GH-N gene transcripts and the pituitary transcription factor Pit-1b.
Human neutrophils express GH-N gene transcripts and the pituitary transcription factor Pit-1b.
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人类中性粒细胞表达 GH-N 基因转录本和垂体转录因子 Pit-1b。
DOI：
10.1210/endo.138.10.5568
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发表时间：
1997
期刊：
影响因子：
4.8
通讯作者：
E. Hooghe
中科院分区：
文献类型：
--
作者：
Ron Kooijman;D. Berus;A. Malur;Mireille Delhase;E. Hooghe
由于GH刺激粒细胞的发育和功能，我们研究了GH在粒细胞亚群中的表达。通过免疫细胞化学，25 +/- 7%的人中性粒细胞显示表达免疫反应性GH，而嗜酸性粒细胞为阴性。逆转录-聚合酶链反应（RT-PCR）检测中性粒细胞GH mRNA表达。限制性内切酶分析表明，中性粒细胞表达GH-N基因，但不表达GH-V基因。此外，我们通过蛋白质印迹分析证明，中性粒细胞表达垂体转录因子Pit-1的可变剪接变体，命名为Pit-1b。
Since GH stimulates the development and function of granulocytes, we investigated the expression of GH in granulocyte subsets. By immunocytochemistry, 25 +/- 7% of the human neutrophils were shown to express immunoreactive GH, whereas eosinophils were negative. Reversed transcription (RT)-PCR analysis demonstrated GH mRNA in neutrophils. Restriction analysis revealed that neutrophils express the GH-N gene but not the GH-V gene. Furthermore, we demonstrated by western blot analysis that neutrophils express an alternatively spliced variant of the pituitary transcription factor Pit-1, designated Pit-1b.