Protein Fractions with Techno-Functional and Antioxidant Properties from Nannochloropsis gaditana Microalgal Biomass
Protein Fractions with Techno-Functional and Antioxidant Properties from Nannochloropsis gaditana Microalgal Biomass
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DOI：
10.1166/jbmb.2015.1534
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发表时间：
2015-08-01
影响因子：
0.5
通讯作者：
Verdugo, Marcela
中科院分区：
文献类型：
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作者：
Medina, Camila;Rubilar, Monica;Verdugo, Marcela
从简单的食品和饲料生物质生产到有价值的生物精炼产品(沼气、生物乙醇、色素、蛋白质、纤维等)，微藻是一种很有前途的高价值产品来源。因此，来自加迪塔纳微藻的蛋白质提取物和水解物等蛋白质组分代表着一个未开发的机会。脱脂微藻生物质经碱性pH增溶、等电点沉淀后得到蛋白质提取液(PE)，再用木瓜蛋白酶酶解得到蛋白质水解物(PH)。根据蛋白质含量和氨基酸组成与微藻生物量的关系对这些组分进行了表征。还测定了它们的技术官能化性质(乳化和起泡能力)和抗氧化活性(ORAC法)。PE的总蛋白质含量高达65.9%(w/w)，必需氨基酸含量与粮农组织/世卫组织/联合国参考概况相当。以木瓜酶为水解酶，其水解度可达25.4%，多肽的生成(
Microalgae are a promising source of high-value products ranging from simple biomass production for food and feed to valuable biorefinery products (biogas, bioethanol, pigments, proteins, fibers, etc.). Thus, protein fractions such as protein extract and hydrolysate from the Nannochloropsis gaditana microalgal biomass represent an unexploited opportunity. The protein extract (PE) from defatted microalgal biomass was obtained after solubilization at alkaline pH and precipitation at the isoelectric point, and the protein hydrolysate (PH) was generated by enzymatic hydrolysis using papain. These fractions were characterized according to their protein content and amino acid composition in relation to the microalgal biomass. Their techno-functional properties (emulsifying and foaming capacity) and antioxidant activity (ORAC method) were also determined. PE showed a high total protein content of 65.9% w/w and a content of essential amino acids comparable to the FAO/WHO/UN reference profile. The emulsifying capacity for PE was greater than 50% and the emulsion was stable after 24 h. Using papain as the enzyme for hydrolysis of PE, a degree of hydrolysis (DH) of 25.4% and the generation of peptides (