An immunological determinant of RNase P protein is conserved between Escherichia coli and humans.
An immunological determinant of RNase P protein is conserved between Escherichia coli and humans.
复制标题
RNase P 蛋白的免疫决定簇在大肠杆菌和人类之间是保守的。
DOI：
10.1073/pnas.86.22.8717
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发表时间：
1989
影响因子：
11.1
通讯作者：
Altman,S
中科院分区：
文献类型：
--
作者：
Mamula,MJ;Baer,M;Craft,J;Altman,S
RNase P是一种具有RNA和蛋白质亚基的酶，在原核生物和真核生物中切割tRNA前体分子以形成成熟tRNA的5'末端。针对大肠杆菌RNase P的蛋白亚基C5蛋白制备的兔抗体结合来自大肠杆菌的RNase P蛋白。大肠杆菌和枯草芽孢杆菌的免疫印迹和固相免疫测定。这些兔抗C5抗体也结合来自人（HeLa）细胞的RNase P制剂中的蛋白质（Mr约40，000），并耗尽来自人和大肠杆菌的RNase P制剂的酶活性。coli细胞。最后，兔抗C5抗体从人细胞的粗提物中免疫沉淀含有H1 RNA的核糖核蛋白复合物，H1 RNA是人RNase P的假定RNA组分。这些结果表明，来自E. coli RNase P和来自人细胞的RNase P的蛋白组分。
RNase P, an enzyme with RNA and protein subunits, cleaves tRNA precursor molecules to form the 5' termini of mature tRNAs in both prokaryotes and eukaryotes. Rabbit antibodies made against the protein subunit, C5 protein, of Escherichia coli RNase P bound RNase P protein from E. coli and Bacillus subtilis in immunoblots and solid-phase immunoassays. These rabbit anti-C5 antibodies also bound a protein (Mr approximately 40,000) in preparations of RNase P from human (HeLa) cells and depleted the enzymatic activity from preparations of RNase P from both human and E. coli cells. Finally, rabbit anti-C5 antibodies immunoprecipitated from crude extracts of human cells a ribonucleoprotein complex containing H1 RNA, the putative RNA component of human RNase P. These results show that an antigenic determinant is shared by C5 protein from E. coli RNase P and a protein component of RNase P from human cells.