CO2Fractional Laser Combined with 5-Fluorouracil Ethosomal Gel Treatment of Hypertrophic Scar Macro-, Microscopic, and Molecular Mechanism of Action in a Rabbit Animal Model
CO2Fractional Laser Combined with 5-Fluorouracil Ethosomal Gel Treatment of Hypertrophic Scar Macro-, Microscopic, and Molecular Mechanism of Action in a Rabbit Animal Model
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CO(2)点阵激光联合5-氟尿嘧啶醇糖体凝胶治疗兔动物模型中肥厚性疤痕的宏观、微观和分子作用机制
DOI：
10.1089/rej.2019.2204
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发表时间：
2020-07-23
影响因子：
2.6
通讯作者：
Biskup, Ewelina
中科院分区：
文献类型：
--
作者：
Huang, Jun;Chen, Jun;Biskup, Ewelina
增生性瘢痕（HS）的治疗迄今为止一直是一个临床挑战。本实验观察了CO2分数激光联合5-氟尿嘧啶醇质体凝胶（5-FU EG）对兔HS模型的治疗作用。在兔耳腹侧制作标准化瘢痕模型，分为对照组（不干预）、EG治疗组、激光治疗组和激光+5-FU EG联合治疗组。进行临床宏观和H& E染色显微镜观察以评估HS改善。实时荧光定量PCR检测I、III型胶原、转化生长因子β 1（TGF-β 1）和白细胞介素6（IL-6）的mRNA水平。14 d后，H&E染色显示治疗组HS厚度明显低于对照组，激光治疗组和联合治疗组HS厚度明显低于EG治疗组。I型和III型胶原蛋白、TGF-β 1的mRNA水平在所有治疗组中均显著较低，而IL-6在第14天在激光治疗组中最高。与单纯5-FU EG治疗相比，CO2激光联合治疗的宏观和微观效果更好。抑制I型和III型胶原、TGF-β 1是可能的潜在作用机制，而IL-6的功能仍有待进一步研究。本研究提示5-FU EG与激光联合治疗及单纯激光治疗效果上级5-FU EG单药治疗。HS改善的机制与减少胶原I/III和抑制TGF-β 1表达有关。
The treatment of hypertrophic scar (HS) has thus far been a clinical challenge. We evaluated the therapeutic effect of CO(2)fractional laser combined with 5-fluorouracil ethosomal gel (5-FU EG) in rabbit HS model. HS model was established as standardized scars on the ventral surface of rabbit ears, divided into four groups: control (no intervention), EG treatment, laser treatment, and combined treatment group (laser plus 5-FU EG). Clinical macroscopic and H&E-stained microscopic observations were conducted to assess HS improvement. The mRNA levels of types I and III collagen, transforming growth factor-beta 1 (TGF-beta 1), and interleukin-6 (IL-6) were detected by real-time PCR. After 14 days, H&E staining shows that the thickness of HS in treatment groups was significantly lower compared with the control group, and the thickness in laser treatment group and combined treatment group was significantly lower compared with the EG treatment group. The mRNA levels of types I and III collagen, TGF-beta 1 were significantly low in all treatment groups, whereas IL-6 was highest in the laser treatment group at day 14. The macro- and microscopic effects of the combined and CO(2)fractional laser treatment were better compared with 5-FU EG only. Inhibition of types I and III collagen, TGF-beta 1 are the possible underlying mechanism of action, whereas the function of IL-6 remains to be further studied. Our study suggests that the effect of combined 5-FU EG and laser, as well as laser-only treatment are superior to 5-FU EG monotreatment. The mechanism of HS improvement is related to reduction of collagen I/III and the inhibition of TGF-beta 1 expression.