A novel nitrite biosensor based on the direct electrochemistry of hemoglobin immobilized on MXene-Ti3C2
A novel nitrite biosensor based on the direct electrochemistry of hemoglobin immobilized on MXene-Ti3C2
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DOI：
10.1016/j.snb.2015.04.090
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发表时间：
2015-10-31
影响因子：
8.4
通讯作者：
Zhu, Zhenfeng
中科院分区：
文献类型：
--
作者：
Liu, Hui;Duan, Congyue;Zhu, Zhenfeng
合成了一种新型类石墨烯纳米材料二维层状Ti 3 C2基材料（MXene-Ti 3 C2），并将其用于固定血红蛋白（Hb），制备了无介体生物传感器。用扫描电镜和X射线衍射对MXene-Ti 3C 2的形貌和结构进行了表征。光谱和电化学测试结果表明，MXene-Ti 3C 2是一种优良的氧化还原蛋白固定化基质，具有良好的生物活性和稳定性。由于MXene-Ti_3C_2的大比表面积和高电导率，促进了Hb的直接电子转移，所制备的生物传感器对亚硝酸根的检测表现出良好的性能，具有0.5-11800 μ M的宽线性范围，以及0.12 μ M的极低检测限。这一工作表明MXene-Ti 3C 2是一种潜在的高效酶固定化候选材料，在环境分析中具有广阔的应用前景。(C)2015爱思唯尔B. V.保留所有权利。
Two-dimensional layered Ti3C2-based material (MXene-Ti3C2), which is a new graphene-like nanomaterials, has been synthesized and then used to immobilize hemoglobin (Hb) to fabricate a mediator-free biosensor. The morphology and structure of MXene-Ti3C2 were characterized by scanning electronmicroscopy and X-ray diffraction. Spectroscopic and electrochemical results revealed that MXene-Ti3C2 is an excellent immobilization matrix with biocompatibility for redox protein, affording good protein bioactivity and stability. Due to the large surface area and the high conductivity of MXene-Ti3C2, the direct electron transfer of Hb is facilitated and the prepared biosensors displayed good performance for the detection of nitrite with a wide linear range of 0.5-11800 mu M, as well as an extremely low detection limit of 0.12 mu M. This work suggests that MXene-Ti3C2 is a potential candidate for efficient enzyme immobilization and promising in environmental analysis. (C) 2015 Elsevier B.V. All rights reserved.