Antimicrobial activity of the crude extract, fractions and compounds from stem bark of Ficus ovata (Moraceae)
Antimicrobial activity of the crude extract, fractions and compounds from stem bark of Ficus ovata (Moraceae)
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DOI：
10.1016/j.jep.2009.05.003
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发表时间：
2009-07-30
影响因子：
5.4
通讯作者：
Ngadjui, Bonaventure Tchaleu
中科院分区：
文献类型：
--
作者：
Kuete, Victor;Nana, Frederic;Ngadjui, Bonaventure Tchaleu
研究目的：本研究旨在研究卵叶榕茎皮甲醇提取物（FOB）、级分（FOB 1 -6）和生物导向分级分离后分离的化合物[3-Friedelanone（1）、蒲公英醇乙酸酯（2）、白桦脂酸（3）、油酸（4）、2-羟基异戊烯基丙酮（5）、6、7-（2-异丙烯基呋喃并）-5，2，4-三羟基甘草酸（6）、木豆素（7）和原儿茶酸（8）]。采用微量稀释法测定其对真菌的最低抑菌浓度（MIC）和最低杀菌浓度（MMC结果：MIC测定结果表明，粗提物（FOB）、级分FOB 2和FOB 4以及化合物5对所研究的全部微生物都有活性。其他样品显示出选择性活性，级分FOB 1。FOB 3和FOB 5对50%的测试微生物物种有活性，而FOB 6对40%有活性。化合物8、6、2和7分别抑制了80%、70%、50%和20%的微生物生长。用粗提物观察到的最低MIC值（156 μ g/ml）记录在粪链球菌、白色念珠菌和奥杜氏小孢子菌上。用FOB 4观察到级分的相应值（39 μ g/ml）对抗金黄色葡萄球菌，而用化合物8观察到测试化合物的相应值（10 μ g/ml）对抗奥杜氏小孢子菌。MMC测定的结果表明，大多数的测试样品对所研究的微生物的杀灭效果可以预期。结论：总体结果提供了证据，所研究的植物提取物，以及一些分离的化合物可能是新的抗菌药物的潜在来源。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Aim of the study: This study was designed to investigate the antimicrobial activities of the methanol extracts from the stem bark of Ficus ovata (FOB), fractions (FOB1-6) and compounds isolated following bio-guided fractionation [3-friedelanone (1), taraxeryl acetate (2), betulinic acid (3), oleanoic acid (4), 2-hydroxyisoprunetin (5), 6,7-(2-isopropenyl furo)-5.2.4-trihydroxyisoflavone (6), Cajanin (7) and protocatechuic acid (8)].Materials and Methods: The micro-dilution method was used for the determination of the minimal inhibition concentration (MIC) and the minimal microbicidal concentration (MMC) against fungi (two species), Gram-positive (three species) and Gram-negative bacteria (five species).Results: The results of the MIC determinations indicated that the crude extract (FOB), fractions FOB2 and FOB4 as well as compound 5 were active on the entire studied organisms. Other samples showed selective activity, fractions FOB1. FOB3 and FOB5 being active against 50% of the tested microbial species while FOB6 was active on 40%. Compounds 8, 6, 2 and 7 prevented the growth of 80%, 70%, 50% and 20% of the organisms respectively. The lowest MIC value (156g/ml) observed with the crude extract was recorded on Streptococcus faecalis, Candida albicans and Microsporum audouinii. The corresponding value for fractions (39 mu g/ml) was noted with FOB4 against Staphylococcus aureus, while that of the tested compounds (10 mu g/ml)was observed with compound 8 on Microsporum audouinii. The results of the MMC determination suggested that the cidal effect of most of the tested samples on the studied microorganisms could be expected.Conclusions: The overall results provided evidence that the studied plant extract, as well as some of the isolated compounds might be potential sources of new antimicrobial drug. (C) 2009 Elsevier Ireland Ltd. All rights reserved.