Metal ion mediated molecularly imprinted polymer for selective capturing antibiotics containing beta-diketone structure
Metal ion mediated molecularly imprinted polymer for selective capturing antibiotics containing beta-diketone structure
复制标题
金属离子介导的分子印迹聚合物选择性捕获含有β-二酮结构的抗生素
DOI：
10.1016/j.chroma.2010.10.097
复制
发表时间：
2010-12-24
影响因子：
4.1
通讯作者：
Wu, Jianmin
中科院分区：
文献类型：
--
作者：
Qu, Shanshan;Wang, Xiaobo;Wu, Jianmin
以衣康酸（ITA）和环丙沙星（CIP）分别作为功能单体和模板分子，合成了一种针对喹诺酮类（Qs）和四环素类（TCs）的新型分子印迹聚合物（MIP）。对影响MIP整体性能的因素进行了研究，结果表明Fe3+离子在具有高分子印迹效应的MIP的形成中起着至关重要的作用。同时，单体的螯合能力、模板分子的种类以及单体与模板的摩尔比也对所得MIP的性能有影响。循环伏安法证实，在Fe3+离子的参与下，聚合前可形成ITA-Fe3+-CIP三元配合物。静态和动态结合实验表明，与常用单体甲基丙烯酸（MAA）制备的传统MIP相比，新型MIP显示出显着增强的分子印迹效果和更高的对目标化合物的特异性吸附能力。 MIP 已成功用作固相萃取 (SPE) 吸附剂，用于从地表水样中富集含有 β-二酮结构的广谱抗生素。 HPLC 检测表明，这些加标抗生素的回收率很高 (78.6-113.6%)，而 MIP 柱上非结构相关药物肾上腺素 (EP) 和多巴胺 (DOPA) 的回收率非常低 (4.7-7.6%)。结果表明，通过本工作提出的策略制备的MIP可以特异性地靶向一系列含有β-二酮结构的结构相关的抗生素。 (C) 2010 Elsevier B.V. 保留所有权利。
A new molecularly imprinted polymer (MIP) targeting to quinolones (Qs) and tetracyclines (TCs) was synthesized using itaconic acid (ITA) and ciprofloxacin (CIP) as a functional monomer and template molecule, respectively. Factors affecting the overall performance of MIP were investigated, and the results showed that Fe3+ ion play a vital role in the formation of MIP with high molecular imprinting effect. Meanwhile, the chelating ability of monomer, species of template molecule, as well as the molar ratio of monomer and template also contribute to the performance of the obtained MIP. Cyclic voltammetry verified that, with the participation of Fe3+ ions, a ternary complex of ITA-Fe3+-CIP could be formed before polymerization. Compared with conventional MIP prepared from commonly used monomer, methacrylic acid (MAA), the new MIP show significantly enhanced molecular imprinting effect and higher capacity for specific adsorption of target compounds as revealed by static and dynamic binding experiments. The MIP was successfully used as solid-phase extraction (SPE) adsorbent for enriching a broad spectrum of antibiotics containing beta-diketone structure from surface water sample. HPLC detection showed that high recovery rate (78.6-113.6%) was found in these spiked antibiotics, whereas recovery rate for the non structurally related drugs, epinephrine (EP) and dopamine (DOPA), was very low (4.7-7.6%) on the MIP cartridges. The results demonstrate that the MIP prepared by the strategy proposed in this work, could specifically target to a series of structurally related antibiotics containing beta-diketone structure. (C) 2010 Elsevier B.V. All rights reserved.