Chemopreventive effects of Panax notoginseng and its major constituents on SW480 human colorectal cancer cells.
Chemopreventive effects of Panax notoginseng and its major constituents on SW480 human colorectal cancer cells.
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DOI：
10.3892/ijo.31.5.1149
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发表时间：
2007-11
影响因子：
5.2
通讯作者：
Chong-Zhi Wang;Jing-tian Xie;Bin Zhang;M. Ni;A. Fishbein;H. Aung;S. Mehendale;W. Du;T. He;C. Yuan
中科院分区：
文献类型：
--
作者：
Chong-Zhi Wang;Jing-tian Xie;Bin Zhang;M. Ni;A. Fishbein;H. Aung;S. Mehendale;W. Du;T. He;C. Yuan
在这项研究中，我们评估了三七根提取物 (NGRE) 及其主要成分对 SW480 人结直肠癌细胞的影响。我们采用高效液相色谱法测定了NGRE中主要皂苷的含量。采用细胞计数法评价抗增殖作用，观察浓度相关的抗增殖作用。 1.0 mg/ml 时，NGRE 抑制细胞生长 85.8%（P<0.01），这可能与较高浓度的人参皂苷 Rb1 和 Rg1 有关。三七皂苷R1和人参皂苷Rg1、Rb1对细胞具有抗增殖作用。我们通过测量 [3H]-胸苷掺入来测试 NGRE 对 DNA 合成的影响。 NGRE 在 0.5 和 1 mg/ml 浓度下诱导细胞凋亡。用300μM三七皂苷R1、人参皂苷Rg1和Rb1处理两天，显着增加细胞凋亡。细胞周期和细胞周期蛋白A检测显示NGRE将细胞阻滞在合成阶段并显着增加细胞周期蛋白A的表达。 NGRE 还增强了两种化疗药物 5-氟尿嘧啶和伊立替康的作用。与单独使用化疗药物相比，NGRE 和 5-氟尿嘧啶（或伊立替康）联合治疗的细胞生长减少更多，这表明三七可以减少达到预期效果所需的 5-氟尿嘧啶（或伊立替康）剂量。需要进一步的体内和人体试验来测试三七是否是一种有价值的具有临床有效性的化疗佐剂。
In this study, we evaluated the effects of Panax notoginseng root extract (NGRE) and its major constituents on SW480 human colorectal cancer cells. We used high performance liquid chromatography to determine the contents of major saponins in NGRE. The anti-proliferative effects were evaluated by the cell counting method, and concentration-related anti-proliferative effects were observed. At 1.0 mg/ml, NGRE inhibited cell growth by 85.8% (P<0.01), probably linked to the higher concentration of ginsenosides Rb1 and Rg1. The pharmacologic activities of notoginsenoside R1 and ginsenosides Rg1 and Rb1 on the cells were antiproliferative. We tested the effects of NGRE on DNA synthesis by measuring [3H]-thymidine incorporation. NGRE induced cell apoptosis at 0.5 and 1 mg/ml. Two-day treatment with 300 microM of notoginsenoside R1, ginsenosides Rg1 and Rb1 increased cell apoptosis significantly. Cell cycle and cyclin A assay showed that NGRE arrested cells in the synthesis phase and increased the expression of cyclin A remarkably. NGRE also enhanced the actions of two chemotherapeutic agents, 5-fluorouracil and irinotecan. Cell growth decreased more with the combined treatment of NGRE and 5-fluorouracil (or irinotecan) than with the chemotherapy agent applied alone, suggesting that notoginseng can reduce the dose of 5-fluorouracil (or irinotecan) needed to achieve desired effects. Further in vivo and human trials are warranted to test whether notoginseng is a valuable chemo-adjuvant with clinical validity.