Porous Particle-Reinforced Bioactive Gelatin Scaffold for Large Segmental Bone Defect Repairing
Porous Particle-Reinforced Bioactive Gelatin Scaffold for Large Segmental Bone Defect Repairing
复制标题
用于修复大节段骨缺损的多孔颗粒增强生物活性明胶支架
DOI：
10.1021/acsami.7b19010
复制
发表时间：
2018-02-28
影响因子：
9.5
通讯作者：
Qiu, Dong
中科院分区：
文献类型：
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作者：
Cui, Yang;Zhu, Tengjiao;Qiu, Dong
大段骨缺损的修复仍是临床上的一大挑战，适用于此目的的合成骨移植物仍有很高的需求。本文以生物活性空心纳米粒子和交联明胶为主要原料，制备了亲水性复合支架（生物活性空心粒子凝胶支架）。生物活性纳米颗粒具有多孔结构以及高比表面积;因此，它们与明胶强烈相互作用，以克服亲水性聚合物支架通常面临的溶胀问题。通过这种组合，这些BHP凝胶支架显示出与松质骨相似的多孔结构和机械性能。它们在体外也显示出优异的生物活性和细胞生长促进性能。在大鼠股骨模型上对其中最好的，即10 BHP-凝胶支架进行体内评价，其中发现在12周内，5 mm节段性骨缺损几乎愈合并形成新的骨组织，同时支架本身降解。因此，10 BHP-凝胶支架有可能成为一种有潜力的大段骨缺损修复骨移植材料。
Large segmental bone defect repairing remains a big challenge in clinics, and synthetic bone grafts suitable for this purpose are still highly demanded. In this article, hydrophilic composite scaffolds (bioactive hollow particle (BHP) gel scaffold) composed of bioactive hollow nano particles and cross-linked gelatin have been developed. The bioactive nanoparticles have a porous structure as well as high specific surface area; thus, they interact strongly with gelatin to overcome the swelling problem that a hydrophilic polymer scaffold will usually face. With this combination, these BHP gel scaffolds showed porous structure and mechanical properties similar to those of the cancellous bone. They also showed excellent bioactivity and cell growth promotion performance in vitro. The best of them, namely, 10BHP-gel scaffold, was evaluated in vivo on a rat femur model, where it was found that the 5 mm segmental bone defect almost healed with new bone tissue formed in 12 weeks and the scaffold itself degraded at the same time. Thus, 10BHP-gel scaffold may become a potential bone graft for large segmental bone defect healing in the future.