Hedgehog signaling is essential for normal wound healing
Hedgehog signaling is essential for normal wound healing
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DOI：
10.1111/j.1524-475x.2008.00430.x
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发表时间：
2008-11-01
影响因子：
2.9
通讯作者：
Saadeh, Pierre B.
中科院分区：
文献类型：
--
作者：
Le, Huong;Kleinerman, Rebecca;Saadeh, Pierre B.
刺猬家族的形态原(sonic[shh]，印度刺猬和沙漠刺猬)是胚胎生长和组织模式的中央调节因子。尽管最近的研究表明Shh参与了出生后组织的修复和发育，但缺乏确凿的证据。在这里，我们通过检测Shh信号级联的特异性抑制剂环多巴胺对组织修复的影响，证明了Shh在伤口修复中的重要性。利用小鼠夹板切除创伤模型，减轻这种皮肤松弛的啮齿动物的伤口收缩，我们建立了所有措施(伤口闭合、上皮化、肉芽形成、血管形成和增殖)，当Shh信号被干扰时，伤口愈合受到严重损害。由于Shh的胚胎分裂与明显的表型缺陷相关，我们的发现促使我们研究Shh信号在出生后条件下与不受调控的伤口愈合相关的潜在作用。
The hedgehog family of morphogens (sonic [Shh], Indian, and desert hedgehog) are central regulators of embryologic growth and tissue patterning. Although recent work implicates Shh in postnatal tissue repair and development, conclusive evidence is lacking. Here, we demonstrated the importance of Shh in wound repair, by examining the effects of cyclopamine, a specific inhibitor of the Shh signaling cascade, on tissue repair. Using a murine-splinted excisional wound model, which attenuates wound contraction in this loose-skinned rodent, we established that, by all measures (wound closure, epithelialization, granulation formation, vascularity, and proliferation), wound healing was profoundly impaired when Shh signaling was disrupted. Because embryonic disruption of Shh is associated with distinct phenotypic defects, our findings invite investigation of the potential role of Shh signaling under postnatal conditions associated with disregulated wound healing.