ISOLATION OF A CANDIDATE REPRESSOR ACTIVATOR, NF-E1 (YY-1, DELTA), THAT BINDS TO THE IMMUNOGLOBULIN KAPPA-3' ENHANCER AND THE IMMUNOGLOBULIN HEAVY-CHAIN MU-E1 SITE
ISOLATION OF A CANDIDATE REPRESSOR ACTIVATOR, NF-E1 (YY-1, DELTA), THAT BINDS TO THE IMMUNOGLOBULIN KAPPA-3' ENHANCER AND THE IMMUNOGLOBULIN HEAVY-CHAIN MU-E1 SITE
复制标题
DOI：
10.1073/pnas.88.21.9804
复制
发表时间：
1991-11-01
影响因子：
11.1
通讯作者：
ATCHISON, ML
中科院分区：
文献类型：
--
作者：
PARK, K;ATCHISON, ML
我们已经确定，免疫球蛋白kappa-3‘增强子(kappa-E3’)活性的发育控制是正负作用元件共同影响的结果。我们发现kappa-E3‘增强子的中心核心在前B细胞阶段是活跃的，但受到侧翼负作用元件的抑制。负作用序列在位置和方向上抑制增强子的活性。在前B细胞阶段以独立方式进行。我们已经分离到一个人的编码锌指蛋白(NF-E1)的cDNA克隆，它与kappa-E3‘增强子的负作用片段相结合。该蛋白还与免疫球蛋白重链增强子MU-E1结合。NF-E1由与YY-1蛋白相同的基因编码，YY-1蛋白与腺相关病毒P5启动子结合。核因子-E1也是小鼠Delta蛋白的人类同系物，它与核糖体蛋白基因启动子结合。预测的氨基酸序列包含转录激活因子和转录抑制因子的特征。与该基因的共转染研究表明，它能抑制基本启动子的活性。讨论了该蛋白的表观双重功能。
We have determined that the developmental control of immunoglobulin kappa-3' enhancer (kappa-E3') activity is the result of the combined influence of positive- and negative-acting elements. We show that a central core in the kappa-E3' enhancer is active at the pre-B-cell stage but is repressed by flanking negative-acting elements. The negative-acting sequences repress enhancer activity in a position- and orientation. independent manner at the pre-B-cell stage. We have isolated a human cDNA clone encoding a zinc finger protein (NF-E1) that binds to the negative-acting segment of the kappa-E3' enhancer. This protein also binds to the immunoglobulin heavy-chain enhancer mu-E1 site. NF-E1 is encoded by the same gene as the YY-1 protein, which binds to the adeno-associated virus P5 promoter. NF-E1 is also the human homologue of the mouse delta-protein, which binds to ribosomal protein gene promoters. The predicted amino acid sequence of this protein contains features characteristic of transcriptional activators as well as transcriptional repressors. Cotransfection studies with this cDNA indicate that it can repress basal promoter activity. The apparent dual function of this protein is discussed.