Peripheral nerve tissue engineering: Autologous Schwann cells vs. transdifferentiated mesenchymal stem cells
Peripheral nerve tissue engineering: Autologous Schwann cells vs. transdifferentiated mesenchymal stem cells
复制标题
DOI：
10.1089/ten.2006.12.1451
复制
发表时间：
2006-06-01
影响因子：
--
通讯作者：
Fansa, Hisham
中科院分区：
文献类型：
--
作者：
Keilhoff, Gerburg;Goihl, Alexander;Fansa, Hisham
间充质干细胞（MSCs）被评估为周围神经组织工程的替代来源。将从雄性大鼠培养的间充质干细胞、转分化的间充质干细胞或雪旺细胞移植到失活的自体肌肉导管中，桥接雌性大鼠的2 cm坐骨神经间隙。使用细胞因子的混合物开发了MSC和转化的培养MSC向许旺细胞样细胞的分化潜力。SRY基因的聚合酶链反应证实了移植物中植入细胞的存在。6周后，再生监测临床，组织学，形态计量。以自体神经和无细胞肌肉移植物作为对照。血运重建研究表明，与未分化的MSC相比，转分化的MSC促进了新血管生成，并且不影响巨噬细胞的募集。自体神经移植在所有再生参数中表现出最好的结果。在雪旺细胞组中注意到适当的再生，尽管有限制，但在转分化的MSC组中，而MSC组和无细胞组中的再生受损。结果表明，转分化的MSC植入失活的肌肉移植物能够在一定程度上支持周围神经再生，并提供了新的治疗策略的潜力。
Mesenchymal stem cells (MSCs) were evaluated as an alternative source for tissue engineering of peripheral nerves. MSCs, transdifferentiated MSCs, or Schwann cells cultured from male rats were grafted into devitalized autologous muscle conduits bridging a 2-cm sciatic nerve gap in female rats. The differentiation potential of MSCs and transformed cultivated MSCs into Schwann cell-like cells was exploited using a cocktail of cytokines. Polymerase chain reaction of the SRY gene confirmed the presence of the implanted cells in the grafts. After 6 weeks, regeneration was monitored clinically, histologically, and morphometrically. Autologous nerves and cell-free muscle grafts were used as control. Revascularization studies suggested that transdifferentiated MSCs, in contrast to undifferentiated MSCs, facilitated neo-angiogenesis and did not influence macrophage recruitment. Autologous nerve grafts demonstrated the best results in all regenerative parameters. An appropriate regeneration was noted in the Schwann cell-groups and, albeit with restrictions, in the transdifferentiated MSC groups, whereas regeneration in the MSC group and in the cell-free group was impaired. The results indicate that transdifferentiated MSCs implanted into devitalized muscle grafts are able to support peripheral nerve regeneration to some extent, and offer a potential for new therapeutic strategies.