High-resolution optical coherence tomography-guided laser ablation of surgical tissue
High-resolution optical coherence tomography-guided laser ablation of surgical tissue
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DOI：
10.1006/jsre.1998.5555
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发表时间：
1999-04-01
影响因子：
2.2
通讯作者：
Fujimoto, JG
中科院分区：
文献类型：
--
作者：
Boppart, SA;Herrmann, J;Fujimoto, JG
背景光学相干断层扫描（OCT）是一种紧凑的高速成像技术，它使用红外光来获取微米尺度上的组织横截面图像。由于OCT图像基于组织的光学后向散射特性，因此应使用该技术检测到由于手术激光消融引起的组织光学特性的变化。在这项工作中，我们研究了使用实时OCT成像来指导放置的可行性，并观察了手术激光消融在各种组织类型中的动态。在激光消融之前、期间和之后，以每秒八帧的速度对五种离体大鼠器官组织类型上的超过65个部位进行成像。用在514-nm波长下操作并改变曝光功率和持续时间的同时连续波氩激光进行消融。组织配准是通过显微注射染料，然后进行常规的组织学处理来确认消融部位的形态学。每秒8帧的高速OCT成像允许在许多器官标本中快速组织定向和引导消融。采集速率足够快，可以捕获与激光消融过程中热组织损伤相对应的光学背向散射的动态变化。高分辨率高速OCT引导激光消融和动态变化成像的能力表明其在图像引导外科手术中的作用，例如肿瘤消融。需要进一步的体内研究来证明术中性能。(C)北京：科学出版社.
Background. Optical coherence tomography (OCT) is a compact high-speed imaging technology which uses infrared light to acquire cross-sectional images of tissue on the micrometer scale. Because OCT images are based on the optical backscattering properties of tissue, changes in tissue optical properties due to surgical laser ablation should be detectable using this technique. In this work, we examine the feasibility of using real-time OCT imaging to guide the placement and observe the dynamics of surgical laser ablation in a variety of tissue types.Materials and methods. More than 65 sites on five ex vivo rat organ tissue types were imaged at eight frames per second before, during, and after laser ablation, Ablation was performed with a coincident continuous wave argon laser operating at 514-nm wavelength and varying exposure powers and durations, Following imaging, tissue registration was achieved using microinjections of dye followed by routine histologic processing to confirm the morphology of the ablation site.Results. High-speed OCT imaging at eight frames per second permitted rapid tissue orientation and guided ablation in numerous organ specimens. Acquisition rates were fast enough to capture dynamic changes in optical backscatter which corresponded to thermal tissue damage during laser ablation,Conclusions. The ability of high-resolution highspeed OCT to guide laser ablation and image the dynamic changes suggests a role in image-guided surgical procedures, such as the ablation of neoplasms. Future in vivo studies are necessary to demonstrate performance intraoperatively. (C) 1999 Academic Press.