Production of Substituted Styrene Bioproducts from Lignin and Lignocellulose Using Engineered Pseudomonas putida KT2440
Production of Substituted Styrene Bioproducts from Lignin and Lignocellulose Using Engineered Pseudomonas putida KT2440
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DOI：
10.1002/biot.201900571
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发表时间：
2020-06-09
影响因子：
4.7
通讯作者：
Bugg, Timothy D. H.
中科院分区：
文献类型：
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作者：
Williamson, James J.;Bahrin, Nurfariza;Bugg, Timothy D. H.
阿魏酸是一种可再生的化学物质，存在于来自草如小麦秸秆和甘蔗的木质纤维素中。恶臭假单胞菌能够从植物生物质中释放和代谢阿魏酸。羟基肉桂酰-CoA水合酶-裂解酶基因（ech）的缺失产生了不能利用阿魏酸和对香豆酸的恶臭假单胞菌菌株，其能够从小麦秸秆或甘蔗渣中积累阿魏酸和对香豆酸。该菌株的进一步工程化看到用酚酸脱羧酶padC替代ech，其将对香豆酸和阿魏酸分别转化为4-乙烯基苯酚和调味剂4-乙烯基愈创木酚。含有padC的工程菌株能够从含有木质纤维素或绿色价值Protobind木质素作为原料的培养基中产生4-乙烯基愈创木酚和4-乙烯基苯酚，并且不需要添加外源诱导物分子。还使用变色栓菌漆酶进行4-乙烯基愈创木酚和4-乙烯基儿茶酚苯乙烯产物的生物聚合，以小规模产生“生物聚苯乙烯”材料。
Ferulic acid is a renewable chemical found in lignocellulose from grasses such as wheat straw and sugarcane. Pseudomonas putida is able to liberate and metabolize ferulic acid from plant biomass. Deletion of the hydroxycinnamoyl-CoA hydratase-lyase gene (ech) produced a strain of P. putida unable to utilize ferulic and p-coumaric acid, which is able to accumulate ferulic acid and p-coumaric acid from wheat straw or sugar cane bagasse. Further engineering of this strain saw the replacement of ech with the phenolic acid decarboxylase padC, which converts p-coumaric and ferulic acid into 4-vinylphenol and the flavor agent 4-vinylguaiacol, respectively. The engineered strain containing padC is able to generate 4-vinylguaiacol and 4-vinylphenol from media containing lignocellulose or Green Value Protobind lignin as feedstock, and does not require the addition of an exogenous inducer molecule. Biopolymerization of 4-vinylguaiacol and 4-vinylcatechol styrene products is also carried out, using Trametes versicolor laccase, to generate "biopolystyrene" materials on small scale.