Dec2 Promotes Th2 Cell Differentiation by Enhancing IL-2R Signaling
Dec2 Promotes Th2 Cell Differentiation by Enhancing IL-2R Signaling
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Dec2 通过增强 IL-2R 信号传导促进 Th2 细胞分化
DOI：
10.4049/jimmunol.0900975
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发表时间：
2009-11-15
影响因子：
4.4
通讯作者：
Sun, Bing
中科院分区：
文献类型：
--
作者：
Liu, Zhiduo;Li, Zhenhu;Sun, Bing
Th细胞的分化在不同阶段受到数千个基因的精确调控。在本研究中，我们证明Dec2是属于bHLH（碱性螺旋-环-螺旋）超家族的转录因子，在Th2分化过程中被逐渐诱导，特别是在晚期。逆转录病毒介导的基因转移或转基因操作证明，上调的 Dec2 在 Th2 诱导条件下可以强烈促进 Th2 发育。此外，在 Dec2 转基因小鼠体内也可检测到 Th2 反应的增强。相反，RNA 干扰介导的内源性 Dec2 抑制可能会减弱 Th2 分化。最后，我们表明，Th2 发育增强至少部分是由于 Dec2 引起的 CD25 表达大幅上调，从而导致对 IL-2 刺激的高反应性。免疫学杂志，2009，183：6320-6329。
Th cell differentiation is precisely regulated by thousands of genes at different stages. In the present study, we demonstrate that Dec2, a transcription factor belonging to the bHLH (basic helix-loop-helix) superfamily, is progressively induced during the course of Th2 differentiation, especially at the late stage. The up-regulated Dec2 can strongly promote Th2 development under Th2-inducing conditions, as evidenced by retrovirus-mediated gene transfer or transgenic manipulation. In addition, an enhancement of Th2 responses is also detectable in Dec2 transgenic mice in vivo. Conversely, RNA interference-mediated suppression of endogenous Dec2 could attenuate Th2 differentiation. Finally, we show that the enhanced Th2 development is at least in part due to substantial up-regulation of CD25 expression elicited by Dec2, thereby resulting in hyperresponsiveness to IL-2 stimulation. The Journal of Immunology, 2009, 183: 6320-6329.