Studies on the action mechanism of benzoylurea insecticides to inhibit the process of chitin synthesis in insects: A review on the status of research activities in the past, the present and the future prospects
Studies on the action mechanism of benzoylurea insecticides to inhibit the process of chitin synthesis in insects: A review on the status of research activities in the past, the present and the future prospects
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DOI：
10.1016/j.pestbp.2009.10.001
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发表时间：
2010-06-01
影响因子：
4.7
通讯作者：
Matsumura, Fumio
中科院分区：
文献类型：
--
作者：
Matsumura, Fumio
本文综述了苯甲酰脲类杀虫剂的原型化合物--灭幼脲（DFB）抑制昆虫几丁质合成的分子机理。确定这种杀虫剂作用位点的关键问题是缺乏在无细胞条件下抑制昆虫几丁质合成的体外证明。这个问题得到了解决时，一种方法，使用细胞内囊泡制备新蜕皮美洲大蠊的角质层开发。使用这种方法，它已成为可能，以确定DFB确实抑制N-乙酰葡糖胺纳入昆虫几丁质的过程。最近，在这一领域有了突破，在果蝇中发现了磺酰脲受体（SUR）。该信息有助于确定来自美洲大蠊以及德国小蠊的上述细胞内囊泡制剂中的昆虫SUR可能是DFB引起几丁质合成抑制的实际靶位点。通过使用典型的SUR特异性抑制剂格列本脲作为辅助剂，已经确定了SUR在这种情况下的作用，以帮助这些囊泡的胞吐运动，就像昆虫SUR所属的ABC转运蛋白组的其他成员的情况一样。在这种情况下，DFB和格列本脲均显示通过抑制K.通道，这导致其抑制几丁质合成。(C)2009年由Elsevier Inc.出版
In this review an effort was made to summarize the up to date information on the knowledge on the action mechanism of diflubenzuron (DFB), a prototype chemical for the benzoylurea type insecticides, with respect to its molecular mechanism to inhibit insect chitin synthesis. The key problem in pinpointing the action site of this insecticide has been the lack of in vitro demonstration of its action to inhibit insect chitin synthesis under cell free conditions. This problem was solved when an approach using a intracellular vesicle preparation from the cuticle of newly molted Periplaneta americana was developed. Using this approach it has become possible to identify that DFB indeed inhibits the process of incorporation of N-acetylglucosamine into insect chitin. Recently there has been a breakthrough in this field, when a sulfonylurea receptor (SUR) was identified in Drosophila melanogaster. This information was instrumental in establishing that insect SUR in the above intracellular vesicular preparation from P. americana as well as Blattella germanica is likely the actual target site of DFB to cause inhibition of chitin synthesis. The role of SUR in this case has been determined, by using glibenclamide, a typical SUR specific inhibitor as an aid, to be helping the exocytotic movement of these vesicles as is the case of other members of the group of ABC-transporters to which insect SUR belongs. In this case both DFB and glibenclamide have been shown to cause the depolarization of the vesicle membrane through inhibition of the K. channel, which leads to their inhibition of chitin synthesis. (C) 2009 Published by Elsevier Inc.