Synthesis of a multi-functional DNA nanosphere barcode system for direct cell detection
Synthesis of a multi-functional DNA nanosphere barcode system for direct cell detection
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DOI：
10.1039/c7nr03615a
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发表时间：
2017-10-07
期刊：
影响因子：
6.7
通讯作者：
Lee, Jong Bum
中科院分区：
文献类型：
--
作者：
Han, Sangwoo;Lee, Jae Sung;Lee, Jong Bum
基于核酸的技术已经被应用于许多生物医学应用。DNA作为一种新型的目标探测材料，已被用于构建具有多种结构的条形码系统。本文报道了利用多个功能化核苷酸的滚环扩增(RCA)制备多功能DNA纳米球(DNANS)。带有条形码系统的DNANS被设计为显示用于编码增强信号的荧光，并含有生物素以实现更多功能，包括通过生物素-链霉亲和素(生物素-STA)相互作用进行靶向。在RCA过程中，功能化的脱氧核苷酸三磷酸(DNTPs)被混合在一起，功能部分可以在DNANS上表达。抗表皮生长因子受体抗体(抗EGFR Abs)可以偶联到DNANS上，特异性地靶向癌细胞。作为概念的证明，多功能DNANS条形码作为一种简单的检测方法，通过直接细胞检测来展示其潜力。DNANS条形码为癌细胞的简单快速选择性识别提供了一条新的途径。
Nucleic acid-based technologies have been applied to numerous biomedical applications. As a novel material for target detection, DNA has been used to construct a barcode system with a range of structures. This paper reports multi-functionalized DNA nanospheres (DNANSs) by rolling circle amplification (RCA) with several functionalized nucleotides. DNANSs with a barcode system were designed to exhibit fluorescence for coding enhanced signals and contain biotin for more functionalities, including targeting through the biotin-streptavidin (biotin-STA) interaction. Functionalized deoxynucleotide triphosphates (dNTPs) were mixed in the RCA process and functional moieties can be expressed on the DNANSs. The anti-epidermal growth factor receptor antibodies (anti-EGFR Abs) can be conjugated on DNANSs for targeting cancer cells specifically. As a proof of concept, the potential of the multi-functional DNANS barcode was demonstrated by direct cell detection as a simple detection method. The DNANS barcode provides a new route for the simple and rapid selective recognition of cancer cells.