The stimulation of sugar transport in heart cells grown in a serum-free medium by picomolar concentrations of thyroid hormones: the effects of insulin and hydrocortisone.
The stimulation of sugar transport in heart cells grown in a serum-free medium by picomolar concentrations of thyroid hormones: the effects of insulin and hydrocortisone.
复制标题
通过皮摩尔浓度的甲状腺激素刺激在无血清培养基中生长的心脏细胞中的糖转运：胰岛素和氢化可的松的作用。
作者：
A. Gordon;H. Schwartz;J. Gross
鸡胚心脏细胞在无血清培养基中增殖。它们形成了一个融合的，同步收缩的单层，这与在含血清培养基中生长的肌细胞没有什么不同。通过暴露于生理浓度的T3（1 pM）和T4（10 pM）刺激这些细胞中2-脱氧-D-[1- 3 H]葡萄糖的摄取。放线菌素D和嘌呤霉素没有阻止2-脱氧-D-[1- 3 H]葡萄糖摄取的刺激时，给予T3在整个6小时的孵育期。在胰岛素和氢化可的松都不存在的情况下生长的细胞对T3无反应。200 nM的胰岛素恢复了细胞对0.1 pM T3的敏感性。在生长培养基中加入10 nM氢化可的松可协同增强T3的作用。5 × 10（-6）M细胞松弛素B可完全阻断T3刺激的糖摄取，表明T3的作用与胰岛素类似，是通过葡萄糖转运蛋白转运到质膜。
Chick embryo heart cells were propagated in a defined serum-free medium. They formed a confluent, synchronously contracting monolayer that is not different from myocytes grown in serum containing media. The uptake of 2-deoxy-D-[1-3H]glucose in these cells was stimulated by exposure to physiological concentrations of T3 (1 pM) and T4 (10 pM). Actinomycin-D and puromycin did not block the stimulation of 2-deoxy-D-[1-3H]glucose uptake when given with T3 throughout a 6-h incubation period. Cells grown in the absence of both insulin and hydrocortisone were unresponsive to T3. Insulin at 200 nM restored the sensitivity of the cells to 0.1 pM T3. Addition of 10 nM hydrocortisone to the growth medium enhanced the effects of T3 synergistically. The T3-stimulated sugar uptake was completely blocked by 5 X 10(-6) M cytochalasin B, suggesting that T3 acts, like insulin, by the translocation of glucose transporters to the plasma membrane.