Blocking miR396 increases rice yield by shaping inflorescence architecture
Blocking miR396 increases rice yield by shaping inflorescence architecture
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DOI：
10.1038/nplants.2015.196
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发表时间：
2016-01-01
期刊：
影响因子：
18
通讯作者：
Li, Shaoqing
中科院分区：
文献类型：
--
作者：
Gao, Feng;Wang, Kun;Li, Shaoqing
提高水稻产量以满足全球需求的策略一直是世界各地育种者关注的主要问题。虽然越来越多的与作物产量相关的功能基因已被鉴定，但我们对其相关调控途径的了解有限。以水稻为模型，我们发现，阻断miR396通过直接诱导生长调节因子6(OsGRF6)基因来调节副枝和小穗的发育，从而大大提高了籽粒产量。OsGRF6的上调导致几个直接下游生物分支的协调激活，包括生长素(IAA)生物合成、生长素反应因子以及与枝条和小穗发育相关的转录因子。这项研究描述了一个保守的microRNA(MiRNA)依赖的调控模块，它整合了花序发育、生长素生物合成和信号转导途径，并有可能用于工程高产作物。
Strategies to increase rice productivity to meet the global demand have been the main concern of breeders around the world. Although a growing number of functional genes related to crop yield have been characterized, our understanding of its associated regulatory pathways is limited. Using rice as a model, we find that blocking miR396 greatly increases grain yield by modulating development of auxiliary branches and spikelets through direct induction of the growth regulating factor 6 (OsGRF6) gene. The upregulation of OsGRF6 results in the coordinated activation of several immediate downstream biological clades, including auxin (IAA) biosynthesis, auxin response factors, and branch and spikelet development-related transcription factors. This study describes a conserved microRNA (miRNA)-dependent regulatory module that integrates inflorescence development, auxin biosynthesis and signalling pathways, and could potentially be used in engineering high-yield crop plants.