COMPARATIVE EFFICACY OF PNEUMOCOCCAL NEURAMINIDASE AND PNEUMOLYSIN AS IMMUNOGENS PROTECTIVE AGAINST STREPTOCOCCUS-PNEUMONIAE
COMPARATIVE EFFICACY OF PNEUMOCOCCAL NEURAMINIDASE AND PNEUMOLYSIN AS IMMUNOGENS PROTECTIVE AGAINST STREPTOCOCCUS-PNEUMONIAE
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DOI：
10.1016/0882-4010(88)90007-1
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发表时间：
1988-12-01
影响因子：
3.8
通讯作者：
HANSMAN, D
中科院分区：
文献类型：
--
作者：
LOCK, RA;PATON, JC;HANSMAN, D
从肺炎链球菌培养物中纯化出神经氨酸酶和肺炎链球菌溶血素，并单独或组合使用来免疫幼年小鼠，随后用强毒力肺炎链球菌鼻内攻击这些小鼠。在两项独立试验的每一项中，在用神经氨酸酶免疫的组中观察到存活时间小幅但显着（P < 0.05）的增加（与未免疫小鼠相比），但前提是该酶已用 3.4% (v/v) 甲醛进行预处理。与仅用肺炎球菌溶血素免疫的小鼠相比，存活时间的中位延长显着缩短（P < 0.01）。同时接受甲醛处理的神经氨酸酶和肺炎球菌溶血素的小鼠的中位生存时间与仅接受肺炎球菌溶血素的小鼠没有显着差异。虽然这些发现提供了直接证据，表明神经氨酸酶有助于小鼠肺炎球菌的致病性，但它们表明，在本实验模型中，这种蛋白质作为疫苗成分的价值可能低于肺炎球菌溶血素。
Neuraminidase and pneumolysin were purified from cultures of Streptococcus pneumoniae and used, either singly or in combination, to immunize juvenile mice which were subsequently challenged intranasally with virulent S. pneumoniae. In each of two independent trails, a small but significant (P < 0.05) increase in survival time (compared with that of non-immunized mice) was observed in groups which had been immunized with neuraminidase, but only if the enzyme had been pre-treated with 3.4% (v/v) formaldehyde. The median extension in survival time was significantly less (P < 0.01) than that of mice which had been immunized with pneumolysin alone. The median survival time for mice which had received both formaldehyde-treated neuraminidase and pneumolysin was not significantly different from that of mice which had received pneumolysin alone. While these findings provide direct evidence that neuraminidase contributes to the pathogenicity of the pneumococcus in mice, they suggest that this protein may be of less value than pneumolysin as a vaccine component in the present experimental model.