Human transcription factor TFIIIC2 specifically interacts with a unique sequence in the Xenopus laevis 5S rRNA gene.
Human transcription factor TFIIIC2 specifically interacts with a unique sequence in the Xenopus laevis 5S rRNA gene.
复制标题
人类转录因子 TFIIIC2 与非洲爪蟾 5S rRNA 基因中的独特序列特异性相互作用。
DOI：
10.1128/mcb.9.11.4941-4950.1989
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发表时间：
1989
影响因子：
5.3
通讯作者：
Dasgupta,A
中科院分区：
文献类型：
--
作者：
Fradkin,LG;Yoshinaga,SK;Berk,AJ;Dasgupta,A
Transcription factor TFIIIC2 derived from human cells is required for tRNA-type gene transcription and binds with high affinity to the essential B-box promoter element of tRNA-type genes. Although 5S rRNA genes contain no homology with the tRNA-type gene B box, we show that TFI1IC2 is also required forXenopus laevis5S rRNA gene transcription. TFIIIC2 protected an approximately 30-base-pair (-10 to +18) region of aXenopus5S rRNA gene from DNase I digestion. This region, which spanned the transcription start site, included sequences that are highly conserved among eucaryotic 5S rRNA genes and have no homology with the B-box sequence of tRNA genes. Mutation of the TFIIIC2-binding site reduced transcription of the 5S rRNA gene by a factor of 10 in HeLa cell extracts. Methylation of C residues within the TFIIIC2-binding site interfered with binding of TFIIIC2. These results suggest a role of the TFIIIC2-binding sequence in 5S rRNA gene transcription. In addition, the 5S rRNA gene binding site and the tRNA-type gene B-box sequence did not compete with each other for binding to TFIIIC2 any better than did an unrelated DNA sequence, indicating that TFIIIC2 interacts with 5S rRNA genes and tRNA-type genes through separate DNA-binding domains or polypeptides.
