Focal Subcortical White Matter Lesions Disrupt Resting State Cortical Interhemispheric Functional Connectivity in Mice
Focal Subcortical White Matter Lesions Disrupt Resting State Cortical Interhemispheric Functional Connectivity in Mice
复制标题
局灶性皮质下白质病变破坏小鼠静息状态皮质半球间功能连接
DOI：
10.1093/cercor/bhab134
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Ayata Cenk
中科院分区：
文献类型：
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作者：
Aykan Sanem A;Xie Hongyu;Lai James Han;Zheng Yi;Chung David Y;Kura Sreekanth;Anzabi Maryam;Sugimoto Kazutaka;McAllister Lauren M;Yaseen M Abbas;Boas David A;Whalen Michael J;Sakadzic Sava;Ayata Cenk
胼胝体是大脑中最大的白色物质束，对大脑半球间的连接至关重要。不幸的是，实验性白色物质损伤后的神经认知缺陷是微妙的和可变的，限制了它们的翻译效用。我们研究了静息状态功能连接（RSFC）作为一个替代后，在胼胝体外侧立体定位注射L-NIO诱导的局灶性病变的小鼠。在注射前和注射后第1天和第14天，通过完整颅骨的光学固有信号成像进行RSFC，使用半球间的同伦和基于种子的时间相关图。我们在同一队列中测量了1个月时的病变体积。L-NIO诱导胼胝体局灶性病变。L-NIO后24小时，大脑半球间的同源连通性下降了50%，部分保留了视觉皮层。所有种子均显示与对侧半球的连通性丧失。此外，同侧运动和视觉皮层在同一半球内失去了连通性。假手术小鼠未显示任何病变或连接变化。RSFC成像可靠地检测小鼠胼胝体损伤后长半球间和半球内连接的急性中断。这种非侵入性的方法可以作为功能替代品，以补充白色损伤后的治疗和恢复研究中的神经认知测试。
The corpus callosum is the largest white matter tract and critical for interhemispheric connectivity. Unfortunately, neurocognitive deficits after experimental white matter lesions are subtle and variable, limiting their translational utility. We examined resting state functional connectivity (RSFC) as a surrogate after a focal lesion in the lateral corpus callosum induced by stereotaxic injection of L-NIO in mice. RSFC was performed via optical intrinsic signal imaging through intact skull before and on days 1 and 14 after injection, using interhemispheric homotopic and seed-based temporal correlation maps. We measured the lesion volumes at 1 month in the same cohort. L-NIO induced focal lesions in the corpus callosum. Interhemispheric homotopic connectivity decreased by up to 50% 24 h after L-NIO, partially sparing the visual cortex. All seeds showed loss of connectivity to the contralateral hemisphere. Moreover, ipsilesional motor and visual cortices lost connectivity within the same hemisphere. Sham-operated mice did not show any lesion or connectivity changes. RSFC imaging reliably detects acute disruption of long interhemispheric and intrahemispheric connectivity after a corpus callosum lesion in mice. This noninvasive method can be a functional surrogate to complement neurocognitive testing in both therapeutic and recovery studies after white matter injury.