Cell Encapsulating Biomaterial Regulates Mesenchymal Stromal/Stem Cell Differentiation and Macrophage Immunophenotype
Cell Encapsulating Biomaterial Regulates Mesenchymal Stromal/Stem Cell Differentiation and Macrophage Immunophenotype
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DOI：
10.5966/sctm.2012-0061
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发表时间：
2012-10-01
影响因子：
6
通讯作者：
Kao, Weiyuan John
中科院分区：
文献类型：
--
作者：
Cantu, David Antonio;Hematti, Peiman;Kao, Weiyuan John
骨髓间充质基质/干细胞（MSC）包封在生物基质中可以改善细胞输送，并延长生存和停留时间超过传统的静脉内给药。虽然间充质干细胞调节单核细胞/巨噬细胞（M空集）的免疫表型特性，很少有人知道这种相互作用是如何影响时，间充质干细胞被截留在一种生物材料。此外，细胞包封基质对MSC多能性和对M空集（其渗透生物材料）的影响仍然知之甚少。在这里，我们阐明了这三种方式的相互作用。关于用于MSC包埋的建立的和实验的胶原基生物材料，检查了M空集免疫表型和MSC分化。肿瘤坏死因子-α的分泌在第4天被急性抑制。MSC与M空组共培养表现出减弱的软骨细胞分化，而成骨细胞分化增强。脂肪细胞的分化大大增强了明胶/聚乙二醇为基础的基质内包埋的MSC。更好地理解细胞包封对MSC的分化潜能和免疫调节的影响对于基于MSC的生物材料使能疗法是至关重要的。干细胞翻译医学2012;1：740-749
Bone marrow mesenchymal stromal/stem cell (MSC) encapsulation within a biomatrix could improve cellular delivery and extend survival and residence time over conventional intravenous administration. Although MSCs modulate monocyte/macrophage (Mempty set) immunophenotypic properties, little is known about how such interactions are influenced when MSCs are entrapped within a biomaterial. Furthermore, the impact of the cell-encapsulating matrix on MSC multipotency and on Mempty sets, which infiltrate biomaterials, remains poorly understood. Here we elucidate this three-way interaction. The Mempty set immunophenotype and MSC differentiation were examined with regard to established and experimental collagen-based biomaterials for MSC entrapment. Tumor necrosis factor-alpha secretion was acutely inhibited at 4 days. MSCs cocultured with Mempty sets demonstrated attenuated chondrocyte differentiation, whereas osteoblast differentiation was enhanced. Adipocyte differentiation was considerably enhanced for MSCs entrapped within the gelatin/polyethylene glycol-based matrix. A better understanding of the effect of cell encapsulation on differentiation potency and immunomodulation of MSCs is essential for MSC-based, biomaterial-enabled therapies. STEM CELLS TRANSLATIONAL MEDICINE 2012;1:740-749