Burkholderia nodosa sp nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella
Burkholderia nodosa sp nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella
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DOI：
10.1099/ijs.0.64873-0
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发表时间：
2007-05-01
影响因子：
2.8
通讯作者：
Vandamme, Peter
中科院分区：
文献类型：
--
作者：
Chen, Wen-Ming;de Faria, Sergio M.;Vandamme, Peter
从巴西木本豆科植物糙含羞草（Br 3437（T））和双尖含羞草（Br 3461，Br 3470）的根瘤中分离到3个菌株：Br 3437（T），Br 3461和Br 3470。根据16 S rRNA基因序列相似性，所有菌株先前均显示属于伯克霍尔德氏菌属。一个多相的方法，包括DNA-DNA杂交，PFGE的全基因组DNA图谱，全细胞蛋白质分析，脂肪酸甲酯分析和广泛的生化特性，被用来进一步澄清这些菌株的分类地位，在这里的菌株被分类在一个新的物种，其中的名称Burkholderia nodosa sp。模式菌株Br 3437（T）（=LMG 23741（T）=BCRC 17575（T））是从M.粗糙的。
Three strains, Br3437(T), Br3461 and Br3470, were isolated from nitrogen-fixing nodules on the roots of Mimosa scabrella (Br3437(T)) and Mimosa bimucronata (Br3461, Br3470), both of which are woody legumes native to Brazil. On the basis of 16S rRNA gene sequence similarities, all the strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, PFGE of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia nodosa sp. nov. is proposed. The type strain, Br3437(T) (=LMG 23741(T) =BCRC 17575(T)), was isolated from nodules of M. scabrella.