Aflatoxin B1-contaminated diet disrupts the blood brain barrier and affects fish behavior: Involvement of neurotransmitters in brain synaptosomes
Aflatoxin B1-contaminated diet disrupts the blood brain barrier and affects fish behavior: Involvement of neurotransmitters in brain synaptosomes
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DOI：
10.1016/j.etap.2018.04.003
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发表时间：
2018-06-01
影响因子：
4.3
通讯作者：
Baldisserotto, Bernardo
中科院分区：
文献类型：
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作者：
Baldissera, Matheus D.;Souza, Carine F.;Baldisserotto, Bernardo
众所周知，黄曲霉毒素 B (AFB(1)) 对血脑屏障 (BBB) 内皮细胞的细胞毒性作用与行为功能障碍有关。然而，受 AFB(1) 污染的饮食对银鲶鱼行为的影响仍不清楚。因此，本研究的目的是评估 AFB1 污染的饮食（1177 ppb kg 饲料（-1））是否会损害银鲶鱼的行为，以及是否涉及 BBB 的破坏和大脑突触体中神经递质的改变。与对照组（基础饮食）相比，喂食受 AFB1 污染的饮食的鱼在第 14 天和第 21 天表现出与过度运动相关的行为障碍。第 14 天和第 21 天的神经递质水平也受到影响。与对照组相比，中毒动物的血脑屏障对伊文思蓝染料的渗透性增加，这表明血脑屏障受到破坏。此外，喂食受AFB(1)污染的饲料的鱼，脑突触体中的乙酰胆碱酯酶(AChE)活性增加，而钠钾泵(Na+、K+-ATP酶)的活性降低。基于这一证据，本研究表明，喂食含有 AFB(1) 的饮食的银鲶会表现出与过度运动相关的行为障碍。这种饮食会导致血脑屏障破坏和大脑损伤，这可能会导致行为改变。此外，大脑突触体中AChE和Na+、K+-ATP酶活性的改变可能直接导致这种行为，因为它们可能促进突触功能障碍。此外，过度运动可能被认为是一个重要的宏观标志，表明可能存在 AFB(1) 中毒。
It is known that the cytotoxic effects of aflatoxin B, (AFB(1)) in endothelial cells of the blood-brain barrier (BBB) are associated with behavioral dysfunction. However, the effects of a diet contaminated with AFB(1) on the behavior of silver catfish remain unknown. Thus, the aim of this study was to evaluate whether an AFB1-contaminated diet (1177 ppb kg feed(-1)) impaired silver catfish behavior, as well as whether disruption of the BBB and alteration of neurotransmitters in brain synaptosomes are involved. Fish fed a diet contaminated with AFB1 presented a behavioral impairment linked with hyperlocomotion on days 14 and 21 compared with the control group (basal diet). Neurotransmitter levels were also affected on days 14 and 21. The permeability of the BBB to Evans blue dye increased in the intoxicated animals compared with the control group, which suggests that the BBB was disrupted. Moreover, acetylcholinesterase (AChE) activity in brain synaptosomes was increased in fish fed a diet contaminated with AFB(1), while activity of the sodium-potassium pump (Na+, K+-ATPase) was decreased. Based on this evidence, the present study shows that silver catfish fed a diet containing AFB(1) exhibit behavioral impairments related to hyperlocomotion. This diet caused a disruption of the BBB and brain lesions, which may contribute to the behavioral changes. Also, the alterations in the activities of AChE and Na+, K+-ATPase in brain synaptosomes may directly contribute to this behavior, since they may promote synapse dysfunction. In addition, the hyperlocomotion may be considered an important macroscopic marker indicating possible AFB(1) intoxication.