ATP‐dependent ionic permeability on nuclear envelope in in situ nuclei of Xenopus oocytes
ATP‐dependent ionic permeability on nuclear envelope in in situ nuclei of Xenopus oocytes
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非洲爪蟾卵母细胞原位核膜上 ATP 依赖性离子渗透性
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
Monica Rigatelli
中科院分区：
文献类型：
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作者：
M. Mazzanti;B. Innocenti;Monica Rigatelli
核膜代表细胞质和核质之间的结构和功能屏障。小分子和溶质被动地穿过核膜，而大蛋白质和 RNA 的运输需要代谢能量。使用原位非洲爪蟾卵母细胞核，我们表征了包膜外表面的 ATP 依赖性离子渗透性。 ATP 的存在（但不一定是水解）对于维持通道开放状态至关重要。离子通道的定位仍不清楚。根据形态学和当前动力学数据，我们提出了离子通道和核孔之间的关系。我们试图以这种方式解释离子选择性通道与核膜上大水孔平行存在之间的明显矛盾。在这一假设下，细胞质代谢能量含量的变化将诱导核细胞质被动交换。带电粒子在核膜上的分布和运动可能会影响许多细胞质功能。 ATP 对电流的调节可能在激素刺激、二价离子渗透到细胞核和细胞周期机制中发挥重要作用。-Mazzanti, M.、Innocenti, B.、Rigatelli, M. 非洲爪蟾卵母细胞原位核中核膜上 ATP 依赖性离子渗透性。 FASEB J.8：231-236； 1994年。
The nuclear envelope represents a structural and functional barrier between cytoplasm and nucleoplasm. Small molecules and solutes passively cross the nuclear envelope, whereas the transport of large proteins and RNA requires metabolic energy. Using in situ Xenopus oocyte nuclei, we characterized ATP‐dependent ionic permeabilities on the external surface of the envelope. The presence, but not necessarily the hydrolysis, of ATP is crucial to maintaining the channels in an open state. Localization of the ionic channels is still unclear. From morphologic and current kinetics data, we suggest a relation between the ionic channels and the nuclear pores. We try, in this way, to explain the apparent contradiction between the presence of ion‐selective channels in parallel with large aqueous pores on the nuclear envelope. Under this hypothesis, variations in the metabolic energy content of the cytoplasm would induce nucleocytoplasmic passive exchanges. The distribution and movement of charged particles across the nuclear envelope may influence many cytoplasmic functions. Regulation of the current by ATP could play an important role in hormonal stimulation, divalent ion permeation into the nucleus, and cell cycle mechanisms.—Mazzanti, M., Innocenti, B., Rigatelli, M. ATP‐dependent ionic permeability on nuclear envelope in in situ nuclei of Xenopus oocytes. FASEB J. 8: 231‐236; 1994.
细胞核：结构、功能和动力学。
影响因子：
16.6
作者：
Newport,JW;Forbes,DJ
通讯作者：
Forbes,DJ
胚胎鸡心室细胞自发心跳过程中的Na通道动力学。
影响因子：
3.4
作者：
Mazzanti,M;DeFelice,LJ
通讯作者：
DeFelice,LJ