Complex formation by the human RAD51C and XRCC3 recombination repair proteins
Complex formation by the human RAD51C and XRCC3 recombination repair proteins
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DOI：
10.1073/pnas.111005698
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发表时间：
2001-07-17
影响因子：
11.1
通讯作者：
West, SC
中科院分区：
文献类型：
--
作者：
Masson, JY;Stasiak, AZ;West, SC
在脊椎动物中，RAD 51蛋白是基因重组、DNA修复和细胞增殖所必需的。RAD 51的五个旁系同源物，称为RAD 51 B、RAD 51 C、RAD 51 D、XRCC 2和XRCC 3，已经被鉴定，并且也被证明是重组和基因组稳定性所需的。然而，目前对它们的生物化学性质或精确的生物学功能知之甚少。作为理解RAD 51旁系同源物在重组中的作用的第一步，从杆状病毒感染的昆虫细胞中过表达并纯化人RAD 51 C和XRCC 3蛋白。这两种蛋白质copurify作为一个复杂的，一个属性，反映了他们的内源性协会中观察到的HeLa细胞。纯化的RAD 51 C-XRCC 3复合物结合单链DNA，但不结合双链DNA，以形成蛋白质-DNA网络，该网络已通过电子显微镜观察到。
In vertebrates, the RAD51 protein is required for genetic recombination, DNA repair, and cellular proliferation. Five paralogs of RAD51, known as RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3, have been identified and also shown to be required for recombination and genome stability. At the present time, however, very little is known about their biochemical properties or precise biological functions. As a first step toward understanding the roles of the RAD51 paralogs in recombination, the human RAD51C and XRCC3 proteins were overexpressed and purified from baculovirus-infected insect cells. The two proteins copurify as a complex, a property that reflects their endogenous association observed in HeLa cells. Purified RAD51C-XRCC3 complex binds single-stranded, but not duplex DNA, to form protein-DNA networks that have been visualized by electron microscopy.