Jasmonate-mediated wound signalling promotes plant regeneration
Jasmonate-mediated wound signalling promotes plant regeneration
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茉莉酸介导的伤口信号促进植物再生
DOI：
10.1038/s41477-019-0408-x
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发表时间：
2019-05-01
期刊：
影响因子：
18
通讯作者：
Xu, Lin
中科院分区：
文献类型：
--
作者：
Zhang, Guifang;Zhao, Fei;Xu, Lin
受伤是触发再生的第一个事件(1-4)。然而，伤害信号通路在植物再生中的分子基础在很大程度上还不清楚。我们以前建立了一种方法来研究拟南芥(5，6)的新生根再生(DNRR)，这为分析伤害提供了一个平台。在DNRR过程中，生长素在叶片脱落后被生物合成，并促进细胞命运转换形成根原基(5-7)。在这里，我们展示了茉莉酸(JAs)在DNRR过程中作为一个伤害信号。在叶片脱落后2小时内，叶片外植体中会产生茉莉酸，并激活乙烯反应因子109(ERF109)。ERF109上调邻氨基苯甲酸合成酶αL(Asa1)-生长素生产途径中的色氨酸生物合成基因(8-10)-依赖于组蛋白H3赖氨酸36三甲基化(H3K36me3)(11)在asa1基因座上的预沉积。2小时后，ERF109的活性通过直接与JASMONatEZIM结构域(JAZ)蛋白相互作用而被抑制，以防止对损伤的超敏反应。我们的结果表明，动态JA波与组蛋白甲基化合作，上调生长素产生的脉冲，并促进DNRR对创伤的反应。
Wounding is the first event triggering regeneration(1-4). However, the molecular basis of wound signalling pathways in plant regeneration is largely unclear. We previously established a method to study de novo root regeneration (DNRR) in Arabidopsis thaliana(5,6), which provides a platform for analysing wounding. During DNRR, auxin is biosynthe-sized after leaf detachment and promotes cell fate transition to form the root primordium(5-7). Here, we show that jasmonates (JAs) serve as a wound signal during DNRR. Within 2 h of leaf detachment, JA is produced in leaf explants and activates ETHYLENE RESPONSE FACTOR109 (ERF109). ERF109 upregulates ANTHRANILATE SYNTHASE alpha l (ASA1)-a tryptophan biosynthesis gene in the auxin production pathway(8-10)-dependent on the pre-deposition of SET DOMAIN GROUP8 (SDG8)-mediated histone H3 lysine 36 trimethylation (H3K36me3)(11) on the ASA1 locus. After 2 h, ERF109 activity is inhibited by direct interaction with JASMONATEZIM-DOMAIN (JAZ) proteins to prevent hypersensitivity to wounding. Our results suggest that a dynamic JA wave cooperates with histone methylation to upregulate a pulse of auxin production and promote DNRR in response to wounding.