Effect of Long-Term Consumption of Poultry Egg Products on Growth, Body Composition, and Liver Gene Expression in Zebrafish, Danio rerio.
Effect of Long-Term Consumption of Poultry Egg Products on Growth, Body Composition, and Liver Gene Expression in Zebrafish, Danio rerio.
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DOI：
10.1093/cdn/nzab134
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发表时间：
2021-12
影响因子：
4.8
通讯作者：
Watts SA
中科院分区：
文献类型：
--
作者：
Williams MB;Palmer JW;Chehade SB;Hall AJ;Barry RJ;Powell ML;Harris ML;Sun LY;Watts SA
禽蛋是一种低成本，高蛋白的营养包，可以作为优质饮食的一部分食用。然而，禽蛋产品的消费在历史上是有争议的，这突出了调查长期鸡蛋消费对代谢健康影响的重要性。我们的研究利用斑马鱼，Danio rerio，一种新定义的人类代谢健康模型，来了解食用蛋制品代替其他已知蛋白质来源的代谢后果。配制参考饮食以含有多功能蛋白质与酪蛋白和鱼蛋白水解产物（CON;对照蛋白质来源），这些蛋白质来源在历史上已用于许多参考饮食。这些蛋白质，然后部分取代全蛋（WE;蛋白质和脂质源），鸡蛋白色（EW;蛋白质源），小麦面筋（WG;谷物蛋白质源），或高脂含量的饮食含有一个多功能蛋白质与酪蛋白和鱼蛋白水解产物（HFCON;等氮和isolipidic与WE饮食）在34周的试验（n = 8坦克，10鱼每坦克）。每日喂食开始于幼年生命早期阶段，并终止于生殖成年晚期阶段。对照组与蛋产品饲料的氨基酸组成没有显着变化，但蛋氨酸和赖氨酸明显限制在鱼饲料WG。在终止时，鱼饲料EW作为蛋白质来源的体重增加和身体组成相似的CON饮食喂养。空腹和餐后血糖在任何饮食治疗之间没有差异。使用RNAseq对肝脏转录组的评估显示，喂食CON或WE饮食的斑马鱼之间没有差异基因表达。喂食WG的斑马鱼雄性体重增加较低。长期食用蛋制品促进了代谢健康，与历史上相关的蛋白质相当。这些数据支持蛋制品在动物饮食中保持长期代谢健康的价值。用蛋制品替代标准的膳食蛋白质来源，在斑马鱼的血糖、生长、肥胖或肝脏基因表达方面没有差异。
Poultry eggs are a low-cost, high-protein nutrient package that can be consumed as part of quality diets. However, consumption of poultry egg products is historically contentious, which highlights the importance of investigating impacts of long-term egg consumption on metabolic health. Our study utilized the zebrafish, Danio rerio, a newly defined model of human metabolic health, to understand the metabolic consequence of consuming egg products in lieu of other well-described protein sources. Reference diets were formulated to contain multisource protein with casein and fish protein hydrolysate (CON; control protein sources), the protein sources that have been historically utilized in numerous reference diets. These proteins were then partially replaced with either whole egg (WE; protein and lipid source), egg white (EW; protein source), wheat gluten (WG; cereal protein source), or a high-lipid-content diet containing a multisource protein with casein and fish protein hydrolysate (HFCON; isonitrogenous and isolipidic with the WE diet) in a 34-wk trial (n = 8 tanks, 10 fish per tank). Daily feeding was initiated at the early juvenile life stage and terminated at the late reproductive adult stage. The amino acid composition of control versus egg product diets did not vary substantially, although methionine and lysine were apparently limiting in fish fed WG. At termination, fish fed EW as the protein source had weight gain and body composition similar to those fed the CON diet. Fasting and postprandial blood glucose did not differ between any dietary treatment. Assessment of the liver transcriptome using RNAseq revealed no differential gene expression between zebrafish fed CON or WE diets. Zebrafish fed WG had lower weight gain in males. Long-term consumption of egg products promoted metabolic health equal to that of historically relevant proteins. These data support the value of egg products for maintaining long-term metabolic health in animal diets. Replacement of standard dietary protein sources with egg products results in no differences in blood glucose, growth, adiposity, or liver gene expression in zebrafish.