mTrs130 is a component of a mammalian TRAPPII complex, a Rab1 GEF that binds to COPI-coated vesicles.
mTrs130 is a component of a mammalian TRAPPII complex, a Rab1 GEF that binds to COPI-coated vesicles.
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DOI：
10.1091/mbc.e09-05-0387
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发表时间：
2009-10
影响因子：
3.3
通讯作者：
A. Yamasaki;Shekar Menon;Sidney Yu;Jemima Barrowman;T. Meerloo;V. Oorschot;J. Klumperman;Ayano Satoh;S. Ferro-Novick
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文献类型：
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作者：
A. Yamasaki;Shekar Menon;Sidney Yu;Jemima Barrowman;T. Meerloo;V. Oorschot;J. Klumperman;Ayano Satoh;S. Ferro-Novick
GTTRab 1调节内质网-高尔基体和早期高尔基体交通。鸟嘌呤核苷酸交换因子（GEF）或在分泌途径的这些阶段激活Rab 1的因子目前尚不清楚。Trs 130 p是酵母TRAPPII（转运蛋白颗粒II）复合物的一个亚基，TRAPPII复合物是一种多亚基拴系复合物，是Rab 1同源物Ypt 1 p的GEF。在这里，我们表明，哺乳动物的Trs 130（mTrs 130）是一个类似的TRAPP复合物在哺乳动物细胞中的一个组成部分，我们描述了第一次，这种复杂的膜交通中发挥的作用。mTRAPPII富集在COPI（外壳蛋白I）包被的囊泡和芽上，但不富集在高尔基体上，并且它特异性地激活Rab 1。此外，我们发现，mTRAPPII结合到gamma 1COP，COPI外套接头亚基。通过短发夹RNA耗尽mTrs 130导致高尔基体附近囊泡的增加和早期高尔基体隔室中货物的积累。我们建议mTRAPPII是Rab 1 GEF，将COPI包被的囊泡拴在早期高尔基体膜上。
The GTPase Rab1 regulates endoplasmic reticulum-Golgi and early Golgi traffic. The guanine nucleotide exchange factor (GEF) or factors that activate Rab1 at these stages of the secretory pathway are currently unknown. Trs130p is a subunit of the yeast TRAPPII (transport protein particle II) complex, a multisubunit tethering complex that is a GEF for the Rab1 homologue Ypt1p. Here, we show that mammalian Trs130 (mTrs130) is a component of an analogous TRAPP complex in mammalian cells, and we describe for the first time the role that this complex plays in membrane traffic. mTRAPPII is enriched on COPI (Coat Protein I)-coated vesicles and buds, but not Golgi cisternae, and it specifically activates Rab1. In addition, we find that mTRAPPII binds to gamma1COP, a COPI coat adaptor subunit. The depletion of mTrs130 by short hairpin RNA leads to an increase of vesicles in the vicinity of the Golgi and the accumulation of cargo in an early Golgi compartment. We propose that mTRAPPII is a Rab1 GEF that tethers COPI-coated vesicles to early Golgi membranes.