Long-term estrogen replacement coordinately decreases trkA and β-PPT mRNA levels in dorsal root ganglion neurons
Long-term estrogen replacement coordinately decreases trkA and β-PPT mRNA levels in dorsal root ganglion neurons
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DOI：
10.1006/exnr.1998.6999
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发表时间：
1999-02-01
影响因子：
5.3
通讯作者：
Bufton, SM
中科院分区：
文献类型：
--
作者：
Liuzzi, FJ;Scoville, SA;Bufton, SM
雌激素状态对成年雌性大鼠皮肤敏感性有深远的影响。α-雌激素受体的mRNA和蛋白质的存在下，神经生长因子依赖性，成年雌性大鼠背根神经节（DRG）神经元提出的可能性，雌激素调节皮肤感觉直接作用于初级传入神经元，也许通过改变它们的敏感性，神经生长因子。本研究探讨了长期（90天）每日注射雌激素制剂倍美力（Wyeth-Ayerst，Radnor，PA）对切除卵巢的成年大鼠腰椎DRG高亲和力NGF受体、trkA、mRNA水平和P-前速激肽原（β-PPT）mRNA水平的影响，这些水平已被证明受NGF调节。实验中使用了两种剂量，较高剂量是较低剂量的10倍。这种注射对背根神经节trkA mRNA水平的影响与短期急性雌激素治疗报告的效果相反。目前的数据显示，长期每日雌激素治疗可使trkA mRNA水平降低36%。雌激素治疗90天后，没有明显的剂量效应。此外，如果β-PPT基因表达由NGF通过trkA受体调节，则长期雌激素治疗可使DRG神经元β-PPT mRNA水平降低约30%。与trkA一样，雌激素治疗90天后没有明显的剂量效应。这些数据表明，雌激素调节DRG神经肽基因表达的可能性，也许，皮肤的敏感性，通过调节神经生长因子受体基因的表达。(C)北京：科学出版社.
Estrogen status has profound effects on cutaneous sensitivity in adult female rats. The presence of alpha-estrogen receptor mRNA and protein in NGF-dependent, adult female rat dorsal root ganglion (DRG) neurons raises the possibility that estrogen modulates cutaneous sensation by acting directly on primary afferent neurons, perhaps by altering their sensitivity to NGF. The present study examined the effect of long-term (90 days) daily injections of an estrogen preparation, Premarin (Wyeth-Ayerst, Radnor, PA), to ovariectomized adult rats on lumbar DRG high-affinity NGF receptor, trkA, mRNA levels, and on P-preprotachykinin (beta-PPT) mRNA levels, which have been shown to be regulated by NGF. Two doses were used in the experiments, the higher dose being 10 times that of the lower dose. Such injections had an effect opposite that reported for short-term, acute estrogen treatment on DRG trkA mRNA levels. The current data show that long-term daily estrogen treatment decreases trkA mRNA levels by 36%. After 90 days of estrogen treatment, no dose effect was evident. Moreover, as would be expected if beta-PPT gene expression is regulated by NGF through the trkA receptor, long-term estrogen treatment decreased DRG neuronal beta-PPT mRNA levels by about 30%. As with trkA, there was no dose effect evident after 90 days of estrogen treatment. These data suggest the possibility that estrogen modulates DRG neuropeptide gene expression and, perhaps, cutaneous sensitivity by regulating NGF receptor gene expression. (C) 1999 Academic Press.