Graphene oxide nanosheets in complex with cell penetrating peptides for oligonucleotides delivery
Graphene oxide nanosheets in complex with cell penetrating peptides for oligonucleotides delivery
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DOI：
10.1016/j.bbagen.2017.07.002
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发表时间：
2017-09-01
影响因子：
3
通讯作者：
Langel, Ulo
中科院分区：
文献类型：
--
作者：
Dowaidar, Moataz;Abdelhamid, Hani Nasser;Langel, Ulo
报道了使用细胞穿透肽（CPP; PepFectl 4（PF 14）或PepFectl 4（PF 14）（PF 221））与氧化石墨烯（GO）复合的纳米载体进行基因转染的新策略。进行与CPP和质粒（pGL 3）、剪接校正寡核苷酸（SCO）或小干扰RNA（siRNA）复合的GO。数据显示CPP和寡核苷酸吸附在石墨烯片层的顶部上，而没有观察到GO的粒度的任何变化。GO减轻了CPP的细胞毒性并改善了材料的生物相容性。与pGL 3-CPP（CPP; PF 14或PF 221）相比，GO-pGL 3-CPP（CPP; PF 14或PF 221）的复合物提供2.1-2.5倍的细胞转染增加。GO-SCO-PF 14组装体有效地转染细胞，与使用PF 14的转染相比增加> 10-25倍。GO的浓度在材料纳米毒性和转染效率中起重要作用。这一结果为利用CPP进行基因治疗开辟了新的视野，并为进一步研究提供了一种简单的策略。
A new strategy for gene transfection using the nanocarrier of cell penetrating peptides (CPPs; PepFectl4 (PF14) or PepFectl4 (PF14) (PF221)) in complex with graphene oxide (GO) is reported. GO complexed with CPPs and plasmid (pGL3), splice correction oligonucleotides (SCO) or small interfering RNA (siRNA) are performed. Data show adsorption of CPPs and oligonucleotides on the top of the graphenic lamellar without any observed change of the particle size of GO. GO mitigates the cytotoxicity of CPPs and improves the material biocompatibility. Complexes of GO-pGL3-CPPs (CPPs; PF14 or PF221) offer 2.1-2.5 fold increase of the cell transfection compared to pGL3-CPPs (CPPs; PF14 or PF221). GO-SCO-PF14 assemblies effectively transfect the cells with an increase of > 10-25 fold compared to the transfection using PF14. The concentration of GO plays a significant role in the material nanotoxicity and the transfection efficiency. The results open a new horizon in the gene treatment using CPPs and offer a simple strategy for further investigations.