Community structure of planktonic methane-oxidizing bacteria in a subtropical reservoir characterized by dominance of phylotype closely related to nitrite reducer.
Community structure of planktonic methane-oxidizing bacteria in a subtropical reservoir characterized by dominance of phylotype closely related to nitrite reducer.
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DOI：
10.1038/srep05728
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发表时间：
2014-07-25
影响因子：
4.6
通讯作者：
Fukui M
中科院分区：
文献类型：
--
作者：
Kojima H;Tokizawa R;Kogure K;Kobayashi Y;Itoh M;Shiah FK;Okuda N;Fukui M
甲烷氧化细菌（MOB）通过氧化甲烷获得能量，在淡水生态系统中可能发挥重要作用。本研究对一亚热带水库浮游生物的群落结构进行了调查。通过对16 S rRNA基因的分析研究了细菌群落结构。在用通用引物获得的聚合酶链反应产物的克隆文库中检测到三组遗传学上不同的MOB。这些类群属于γ变形菌纲、α变形菌纲和候选门NC 10。最后一组，由亚硝酸盐还原剂的近亲'Methylomirabilis oxyfera'组成，经常在深水环境的克隆库中检测到。通过对编码颗粒甲烷单加氧酶的pmoA基因的克隆分析，也表明深水中存在3组MOB。M的优势。通过催化报告沉积-荧光原位杂交技术证实了深水中的类oxyfera生物，其中用特异性探针染色的细胞占总微生物细胞的16%。这是第一个研究表明，近亲属的亚硝酸盐还原剂可以是主要组成部分的反硝化MOB社区，可能会影响碳流动的水生态系统。
Methane-oxidizing bacteria (MOB) gain energy from the oxidation of methane and may play important roles in freshwater ecosystems. In this study, the community structure of planktonic MOB was investigated in a subtropical reservoir. Bacterial community structure was investigated through the analysis of the 16S rRNA gene. Three groups of phylogenetically distinct MOB were detected in the clone libraries of polymerase chain reaction products obtained with universal primers. The groups belonged to the class Gammaproteobacteria, the class Alphaproteobacteria, and the candidate phylum NC10. The last group, which consists of close relatives of the nitrite reducer ‘Candidatus Methylomirabilis oxyfera', was frequently detected in the clone libraries of deep-water environments. The presence of 3 groups of MOB in deep water was also shown by a cloning analysis of the pmoA gene encoding particulate methane monooxygenase. The dominance of ‘M. oxyfera'-like organisms in deep water was confirmed by catalyzed reporter deposition–fluorescence in situ hybridization, in which cells stained with a specific probe accounted for 16% of total microbial cells. This is the first study to demonstrate that close relatives of the nitrite reducer can be major component of planktonic MOB community which may affect carbon flow in aquatic ecosystems.
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影响因子：
3.3
作者：
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通讯作者：
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影响因子：
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