Rapid degradation of newly synthesized collagen by primary cultures of adult rat hepatocytes.
Rapid degradation of newly synthesized collagen by primary cultures of adult rat hepatocytes.
复制标题
成年大鼠肝细胞原代培养物快速降解新合成的胶原蛋白。
DOI：
10.1016/0006-291x(80)90337-x
复制
发表时间：
1980
影响因子：
3.1
通讯作者：
Guzelian,PS
中科院分区：
文献类型：
--
作者：
Diegelmann,RF;Cohen,IK;Guzelian,PS
在单层培养的前 24 至 48 小时内，成年大鼠肝细胞合成的放射性羟脯氨酸总量的 95% 以上可溶于 5% TCA。在接下来的 24 小时培养期间，TCA 可溶性羟脯氨酸的量减少至约 40%。同样，大鼠肝癌细胞 (HTC) 也产生大量 TCA 可溶性羟脯氨酸 (48%)。这些发现表明，肝细胞不仅具有合成胶原蛋白的能力，而且还具有降解这种纤维蛋白的能力。
Greater than 95% of the total radioactive hydroxyproline synthesized by adult rat hepatocytes during the first 24 to 48 hours in monolayer culture is soluble in 5% TCA. During the next 24-hour culture period, the amount of TCA-soluble hydroxyproline is reduced to approximately 40%. Likewise, rat hepatoma cells (HTC) also produce a significant amount of TCA-soluble hydroxyproline (48%). These findings suggest that hepatocytes not only have the capacity to synthesize collagen, but also have the ability to degrade this fibrous protein.