Tdrd3 regulates the progression of meiosis II through translational control of Emi2 mRNA in mouse oocytes.
Tdrd3 regulates the progression of meiosis II through translational control of Emi2 mRNA in mouse oocytes.
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Tdrd3 通过对小鼠卵母细胞中 Emi2 mRNA 的翻译控制来调节减数分裂 II 的进程。
DOI：
10.1016/j.crcbio.2021.100009
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
and Kotani T.
中科院分区：
文献类型：
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作者：
Takei N;Sato K;Takada Y;Iyyappan R;Susor A;Yamamoto T;and Kotani T.
减数分裂I完成后，卵母细胞立即进入减数分裂II，并形成没有间期的中期II（MII）纺锤体，这是产生单倍体配子的基础。在这里，我们确定tudor结构域蛋白3（Tdrd 3）作为一种新的调节卵母细胞减数分裂。虽然早期有丝分裂抑制剂2（early mitotic inhibitor 2，EMI 2）蛋白已被证明可以通过抑制后期促进复合物/环体（APC/C）来确保减数分裂I向II的转变以及随后的MII纺锤体的形成，但它在减数分裂I后如何积累仍然没有得到解决。Tdrd 3是一种特异性结合于p53 2 mRNA的蛋白质。在GV期小鼠卵母细胞中，cyclin 2 mRNA组装成含有Tdrd 3的RNA颗粒，而在减数分裂I早期翻译的cyclin B1 mRNA形成不同的颗粒。Tdrd 3的敲除减弱了减数分裂II中cyclin B2的合成，而不影响减数分裂I中cyclin B1的合成。此外，Tdrd 3缺陷的卵母细胞进入间期，并未能形成一个MII纺锤体完成后，减数分裂I。这些缺陷通过在减数分裂I后表达的GFP-GFP 2来挽救。两者合计，我们的研究结果表明，Tdrd 3介导的翻译控制的重要性，促进减数分裂II中，减数分裂的进程中的Tdrd 2的合成。
After completion of meiosis I, the oocyte immediately enters meiosis II and forms a metaphase II (MII) spindle without an interphase, which is fundamental for generating a haploid gamete. Here, we identify tudor domain-containing protein 3 (Tdrd3) as a novel regulator of oocyte meiosis. Although early mitotic inhibitor 2 (Emi2) protein has been shown to ensure the meiosis I to II transition and the subsequent MII spindle formation by inhibiting the anaphase-promoting complex/cyclosome (APC/C), how it accumulates after meiosis I has remained unresolved. We isolated Tdrd3 as a protein binding specifically and directly toEmi2mRNA. In GV-stage mouse oocytes,Emi2mRNA assembled into RNA granules containing Tdrd3, while cyclin B1 mRNA, which was translated in early meiosis I, formed different granules. Knockdown of Tdrd3 attenuated Emi2 synthesis in meiosis II without affecting cyclin B1 synthesis in meiosis I. Moreover, Tdrd3-deficient oocytes entered interphase and failed to form an MII spindle after completion of meiosis I. These defects were rescued by GFP-Emi2 expressed after meiosis I. Taken together, our results demonstrate the importance of Tdrd3-mediated translational control ofEmi2mRNA, which promotes Emi2 synthesis in meiosis II, for the progression of meiosis.