Dendrobium candidum protects against diabetic kidney lesions through regulating vascular endothelial growth factor, Glucose Transporter 1, and connective tissue growth factor expression in rats
Dendrobium candidum protects against diabetic kidney lesions through regulating vascular endothelial growth factor, Glucose Transporter 1, and connective tissue growth factor expression in rats
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DOI：
10.1002/jcb.28666
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发表时间：
2019-08-01
影响因子：
4
通讯作者：
Tian, Hua
中科院分区：
文献类型：
--
作者：
Chang, Jingzhi;Zhou, Yuanting;Tian, Hua
糖尿病（DM）仍然是一个严重的健康问题，大约30%的DM患者最终患有糖尿病肾病。外源性保护因子的研究近年来受到广泛关注。本研究旨在探讨铁皮石斛对糖尿病大鼠肾脏的保护作用。首先建立链脲佐菌素诱导的糖尿病大鼠模型，随机分为模型组、DC组（0.2，0.4，0.8 g/kg）和厄贝沙坦组（17.5 mg/kg）。检测各组大鼠的生化指标、病理变化及血管内皮生长因子（VEGF）、葡萄糖转运蛋白-1（GLUT-1）、结缔组织生长因子（CTGF）的表达。结果表明，与模型组相比，DC治疗组和厄贝沙坦组的肾指数、血清肌酐、血尿素氮、24 h尿蛋白定量、VEGF均显著降低，肾脏病理改变明显改善（P < 0.05）。治疗组大鼠肾皮质GLUT-1和CTGF的蛋白和mRNA水平均显著低于未治疗组。提示DC可能通过调节VEGF、GLUT-1和CTGF的表达对糖尿病大鼠肾脏有保护作用。
Diabetes mellitus (DM) remains a great health problem with approximate 30% of patients with DM eventually suffering from diabetic nephropathy. The search for exogenous protective factors has recently received wide attention. The current study aimed to investigate the protective effects of Dendrobium candidum (DC) on kidneys in diabetic rats. Initially, streptozotocin-induced diabetic rats were established and randomly divided into the model group, DC group (0.2, 0.4, and 0.8 g/kg) and irbesartan group (17.5 mg/kg). The biochemical indexes, pathological changes, and the expressions of vascular endothelial growth factor (VEGF), GLUT-1, and CTGF were examined. It was found that as compared with the model group, the kidney index, serum creatine, blood urea nitrogen, 24-hour urine protein, and VEGF of DC treatment groups were significantly decreased, and pathological changes in kidney were improved in the DC groups and irbesartan group (P < 0.05 for each parameter). The protein and messenger RNA levels of GLUT-1 and CTGF in treatment groups were significantly lower than those in rats' renal cortex without treatment. Our data suggest that DC may protect the kidneys of diabetic rats via regulating expression of VEGF, GLUT-1, and CTGF.