Concentration, compartmentation and metabolic function of intracellular free Mg2+.
Concentration, compartmentation and metabolic function of intracellular free Mg2+.
复制标题
细胞内游离镁的浓度、区室和代谢功能。
DOI：
10.1684/mrh.2006.0067
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发表时间：
2006
影响因子：
3.2
通讯作者：
Theodor Günther
中科院分区：
文献类型：
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作者：
Theodor Günther
细胞内总Mg 2+和游离Mg 2+在细胞器之间和细胞质内被区室化。不同的研究者测量了相同细胞类型的胞质溶胶中不同的[Mg 2 +]i值。差异的一个主要原因是在荧光方法中用于镁furaptra复合物和MgATP时，采用31 P NMR的解离常数的不确定性。在原位条件下测得的糠酸镁和MgATP的KD值比大多数研究者使用的KD值要高。得到了[Mg ~（2+）]，并给出了不同作者使用的KD。综述了细胞内Mg 2+在代谢功能中的作用以及各种效应物对[Mg 2 +]i和[Ca 2 +] i的作用。细胞内Mg 2+可能具有支持效应子诱导机制的许可作用，该机制由Ca 2+作为第二信使介导。
Intracellular total Mg2+ and free Mg2+ are compartmentalized between cell organelles and within the cytosol. Different values of [Mg2+]i in the cytosol of the same cell type were measured by various investigators. A main reason for the differences is the uncertainty of the dissociation constants used for the Mg furaptra complex in the fluorescence method and for MgATP when 31P NMR was employed. The more realistic KD values of Mg furaptra and MgATP measured under in situ conditions are higher than the KDs used by most investigators. The [Mg2+]is obtained and the KDs used by various authors were presented. The role of intracellular Mg2, in metabolic functions and the action of various effectors on [Mg2+]i and [Ca2+] was reviewed. Intracellular Mg2+ may have a permissive role supporting the effector-induced mechanisms that are mediated by Ca2+ as a second messenger.