Hybrid membrane biomaterials from self-assembly in polysaccharide and peptide amphiphile mixtures: controllable structural and mechanical properties and antimicrobial activity
Hybrid membrane biomaterials from self-assembly in polysaccharide and peptide amphiphile mixtures: controllable structural and mechanical properties and antimicrobial activity
复制标题
DOI：
10.1039/c6ra27244d
复制
发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Ruokolainen, J.
中科院分区：
文献类型：
--
作者：
Castelletto, V.;Kaur, A.;Ruokolainen, J.
通过多糖和肽两亲物的协同自组装，制备了基于多尺度分级自组装的具有可调性质的宏观胶囊。不同的制剂可用于在溶液、软胶囊或刚性自立胶囊中产生柔性膜囊。通过将含有藻酸钠的溶液（具有或不具有氧化石墨烯（GO））注射到由含有肽两亲物PA C-16-KKFF（K：赖氨酸，F：苯丙氨酸）的溶液（具有或不具有CaCl 2）组成的基质中来制备样品。将氧化石墨烯添加到混合材料中以调节胶囊的机械性能。将海藻酸钠溶液注射到纯PA基质中提供了溶液中的柔性膜囊，而将NaAlg/GO溶液注射到PA基质中得到软胶囊。或者，通过将NaAlg/GO溶液注入PA + CaCl 2基质溶液中来制备刚性自立式胶囊。通过X射线散射数据的分析，提供了一个全面的洞察胶囊内的层次顺序。一种新的“Langmuir-Blodgett”机制，提出了占的囊和胶囊的形成作为海藻酸盐溶液注入在界面的PA解决方案。该胶囊显示出对革兰氏阳性细菌单核细胞增生李斯特菌（一种重要的人类病原体）的独特抗菌作用。因此，杂化纳米结构胶囊具有显著的生物活性，并且由于其可调的结构和功能特性，可能具有多种其他未来应用。
Macroscopic capsules, with tunable properties based on hierarchical self-assembly on multiple lengthscales, are prepared from the co-operative self-assembly of polysaccharide and peptide amphiphiles. Different formulations can be used to create flexible membrane sacs in solution, soft capsules or rigid free-standing capsules. Samples are prepared by injecting a solution containing sodium alginate, with or without graphene oxide (GO), into a matrix consisting of a solution containing the peptide amphiphile PA C-16-KKFF (K: lysine, F: phenylalanine), with or without CaCl2. Graphene oxide is added to the hybrid materials to modulate the mechanical properties of the capsules. Injection of sodium alginate solution into a pure PA matrix provides a flexible membrane sac in solution, while injection of NaAlg/GO solution into a PA matrix gives a soft capsule. Alternatively, a rigid free-standing capsule is made by injecting a NaAlg/GO solution into a PA + CaCl2 matrix solution. A comprehensive insight into the hierarchical order within the capsules is provided through analysis of X-ray scattering data. A novel "Langmuir-Blodgett" mechanism is proposed to account for the formation of the sacs and capsules as the alginate solution is injected at the interface of the PA solution. The capsules show a unique antibacterial effect specific for the Gram positive bacterium Listeria monocytogenes, which is an important human pathogen. The hybrid nanostructured capsules thus have remarkable bioactivity and due to their tunable structural and functional properties are likely to have a diversity of other future applications.