Th1 cytokines in oral lichen planus
Th1 cytokines in oral lichen planus
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DOI：
10.1034/j.1600-0714.2003.00077.x
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发表时间：
2003-02-01
影响因子：
3.3
通讯作者：
Sugerman, PB
中科院分区：
文献类型：
--
作者：
Khan, A;Farah, CS;Sugerman, PB
背景资料：结果：口腔扁平苔藓（OLP）中上皮下和上皮内的单个核细胞以CD 8（+）为主，细胞因子和细胞因子受体在OLP中的表达和分泌均受细胞因子及其受体的调节。在某些情况下，上皮内CD 8（+）细胞与退化的角质形成细胞相邻。CD 4（+）细胞主要分布于固有层深部，偶见CD 4（+）细胞靠近基底角朊细胞。单核细胞在浅层固有层表达IFN-γ，在基底角质形成细胞附近表达TNF-α。基底角化细胞表达TNF-α作为连续带。TNF R1由单个核细胞和基底和基底上角质形成细胞表达。上皮下浸润中TGF-β 1的表达是可变的，而所有上皮内单核细胞TGF-β 1（-）。OLP中的角质形成细胞TGF-β 1染色较弱。未受刺激的OLP病变T细胞体外分泌IFN-γ。TNF-α刺激下调IFN-γ分泌和上调TNF-α分泌。IL-4，IL-10和TGF-β 1的分泌没有检测到。结论：这些数据表明，T辅助1免疫反应的发展，可能会促进CD 8（+）细胞毒性T细胞活性的OLP。
Background: Cell-mediated immune responses in oral lichen planus (OLP) may be regulated by cytokines and their receptors.Methods: In situ cytokine expression and in vitro cytokine secretion in OLP were determined by immunohistochemistry and ELISA.Resulults: The majority of subepithelial and intraepithelial mononuclear cells in OLP were CD8(+) . In some cases, intraepithelial CD8(+) cells were adjacent to degenerating keratinocytes. CD4(+) cells were observed mainly in the deep lamina propria with occasional CD4(+) cells close to basal keratinocytes. Mononuclear cells expressed IFN-gamma in the superficial lamina propria and TNF-alpha adjacent to basal keratinocytes. Basal keratinocytes expressed TNF-alpha as a continuous band. TNF R1 was expressed by mononuclear cells and basal and suprabasal keratinocytes. There was variable expression of TGF-beta1 in the subepithelial infiltrate while all intraepithelial mononuclear cells were TGF-beta1(-) . Keratinocytes in OLP stained weakly for TGF-beta1. Unstimulated OLP lesional T cells secreted IFN-gammain vitro . TNF-alpha stimulation down-regulated IFN-gamma secretion and up-regulated TNF-alpha secretion. IL-4, IL-10 and TGF-beta1 secretion were not detected.Conclusions: These data suggest the development of a T helper 1 immune response that may promote CD8(+) cytotoxic T-cell activity in OLP.