Molecular analysis of fungal microbiota in samples from healthy human skin and psoriatic lesions
Molecular analysis of fungal microbiota in samples from healthy human skin and psoriatic lesions
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DOI：
10.1128/jcm.00785-06
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发表时间：
2006-08-01
影响因子：
9.4
通讯作者：
Blaser, Martin J.
中科院分区：
文献类型：
--
作者：
Paulino, Luciana C.;Tseng, Chi-Hong;Blaser, Martin J.
银屑病是一种病因不明的常见皮肤病，可能由感染引发，包括真菌感染。由于人类皮肤的真菌群落特征很差，我们的目的是分析健康皮肤和银屑病病变中的真菌微生物群。使用宽范围18 S核糖体DNA（rDNA）和5.8S rDNA/内部转录间隔区2（ITS 2）马拉色菌特异性PCR引物分析了5名健康受试者（前臂屈肌）和3名银屑病患者的25份皮肤样本。宽范围PCR分析表明，大多数微生物类似马拉色菌。对1,374个克隆进行的马拉色菌特异性5.8S/ITS 2分析确定了5个物种和4个未知的马拉色菌类型，可能代表新物种。物种分布似乎主要是宿主特异性的，并且在健康皮肤的不同部位中是保守的。在三名受试者中，马拉色菌菌群组成随时间推移相对稳定。来自健康皮肤和银屑病病变的马拉色菌菌群样本在一名患者中相似，但在另外两名患者中差异很大。这些数据表明马拉色菌生物体在健康人皮肤中占优势，宿主特异性变异，随时间的稳定性，并且迄今为止，没有一致的模式区分银屑病皮肤与健康皮肤。
Psoriasis, a common cutaneous disease of unknown etiology, may be triggered by infections, including those due to fungi. Since the fungal community of human skin is poorly characterized, we aimed to analyze the mycological microbiota in healthy skin and psoriatic lesions. Twenty-five skin samples from five healthy subjects (flexor forearm) and three patients with psoriasis were analyzed using broad-range 18S ribosomal DNA (rDNA) and 5.8S rDNA/internal transcribed spacer 2 (ITS2) Malassezia-specific PCR primers. Broad-range PCR analysis indicated that most organisms resembled Malassezia. Malassezia-specific 5.8S/ITS2 analysis of 1,374 clones identified five species and four unknown phylotypes, potentially representing new species. The species distribution appears largely host specific and conserved in different sites of healthy skin. In three subjects, the Malassezia microbiota composition appeared relatively stable over time. Samples of Malassezia microbiota from healthy skin and psoriatic lesions were similar in one patient but substantially different in two others. These data indicate the predominance of Malassezia organisms in healthy human skin, host-specific variation, stability over time, and as yet, no consistent patterns differentiating psoriatic skin from healthy skin.