Bioavailability Comparison of Nine Bioselenocompounds In Vitro and In Vivo.
Bioavailability Comparison of Nine Bioselenocompounds In Vitro and In Vivo.
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DOI：
10.3390/ijms18030506
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发表时间：
2017-02-26
影响因子：
5.6
通讯作者：
Ogra Y
中科院分区：
文献类型：
--
作者：
Takahashi K;Suzuki N;Ogra Y
硒（Se）在动物体内表现出生物学上的矛盾特性。它是一种必需元素，但当摄入量超过足够的摄入量时，它会变得严重有毒。它的生物、营养和毒理学效应在很大程度上取决于它的化学形式。在这项研究中，我们评估了9种天然存在的硒化合物，或所谓的生物硒化合物，在体内和体外的毒性和生物利用度。亚硒酸盐和硒代胱氨酸的体外毒性高于其他生物硒化合物。在使用Caco-2细胞的体外膜渗透性研究中，硒代蛋氨酸和Se-甲基硒代半胱氨酸比其他生物硒化合物更有效地运输。通过形态分析，从低硒大鼠血清硒蛋白的恢复定量测定了生物硒化合物对营养利用率的影响。与体外研究相比，除三甲基硒离子外，生物硒化合物（包括硒氨基酸、硒糖）和无机硒物种（如亚硒酸盐、硒酸盐和硒氰酸盐）将硒同化为血清硒蛋白的能力无显著差异。这些结果表明，动物可以平等地吸收无机和有机天然存在的硒化合物，除了三甲基硒离子，这是过量硒的尿代谢产物。结果表明，除三甲基硒离子外，其它生物硒化合物的营养有效性相当。
Selenium (Se) shows biologically ambivalent characteristics in animals. It is an essential element but becomes severely toxic when the amount ingested exceeds the adequate intake level. Its biological, nutritional, and toxicological effects are strongly dependent on its chemical form. In this study, we evaluated the toxicity and bioavailability of nine naturally occurring Se compounds, or the so-called bioselenocompounds, in vivo and in vitro. Selenite and selenocystine showed higher toxicity than the other bioselenocompounds in vitro. In an in vitro membrane permeability study using Caco-2 cells, selenomethionine and Se-methylselenocysteine were more efficiently transported than the other bioselenocompounds. The effect of bioselenocompounds on nutritional availability was quantitatively determined from the recovery of serum selenoproteins in Se-deficient rats by speciation analysis. In contrast to the in vitro study, there were no significant differences in the assimilation of Se into serum selenoproteins among the bioselenocompounds, including selenoamino acids, selenosugar, and inorganic Se species, such as selenite, selenate, and selenocyanate, except trimethylselenonium ion. These results indicate that animals can equally assimilate both inorganic and organic naturally occurring selenocompounds except trimethylselenonium ion, which is the urinary metabolite of excess Se. We confirmed that the bioselenocompounds except trimethylselenonium ion had equivalent nutritional availabilities.