Velocity mapping of the aortic flow at 9.4 T in healthy mice and mice with induced heart failure using time-resolved three-dimensional phase-contrast MRI (4D PC MRI)
Velocity mapping of the aortic flow at 9.4 T in healthy mice and mice with induced heart failure using time-resolved three-dimensional phase-contrast MRI (4D PC MRI)
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DOI：
10.1007/s10334-014-0466-z
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发表时间：
2015-08-01
影响因子：
2.3
通讯作者：
Hoerr, Verena
中科院分区：
文献类型：
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作者：
Bovenkamp, Philipp Rene;Brix, Tobias;Hoerr, Verena
目的 在本研究中，我们在 9.4 T 小动物 MRI 系统上建立并验证了时间分辨三维相衬磁共振成像方法 (4D PC MRI)。在此，我们提出了 4D PC MRI 在对横主动脉缩窄 (TAC) 小鼠进行定性和定量血流模式分析方面的可行性。材料和方法使用四点相位编码方案在 9.4 T 下获取血流模型和小鼠心脏的 4D PC FLASH 图像。使用 Bland-Altman 分析将该方法与切片选择性 PC FLASH 和超声进行比较。利用 Voreen 体积渲染软件对高级 3D 流线进行可视化。结果 在体外，4D PC MRI 流动剖面显示随着速度的增加，层流和湍流之间的转变。在体内，升主动脉和肺动脉的4D PC MRI数据通过超声证实，导致R-2 > 0.93的线性回归。 TAC 手术前后的幅度和方向编码流线有很大差异。 结论 4D PC MRI 是在高场小动物扫描仪上测量体内速度的可行工具。与临床测量类似，该方法提供了小鼠心血管血流的完整空间和时间解析数据集，并允许三维血流模式分析。
Objectives In this study, we established and validated a time-resolved three-dimensional phase-contrast magnetic resonance imaging method (4D PC MRI) on a 9.4 T small-animal MRI system. Herein we present the feasibility of 4D PC MRI in terms of qualitative and quantitative flow pattern analysis in mice with transverse aortic constriction (TAC).Materials and methods 4D PC FLASH images of a flow phantom and mouse heart were acquired at 9.4 T using a four-point phase-encoding scheme. The method was compared with slice-selective PC FLASH and ultrasound using Bland-Altman analysis. Advanced 3D streamlines were visualized utilizing Voreen volume-rendering software.Results In vitro, 4D PC MRI flow profiles showed the transition between laminar and turbulent flow with increasing velocities. In vivo, 4D PC MRI data of the ascending aorta and the pulmonary artery were confirmed by ultrasound, resulting in linear regressions of R-2 > 0.93. Magnitude- and direction-encoded streamlines differed substantially pre- and post-TAC surgery.Conclusions 4D PC MRI is a feasible tool for in vivo velocity measurements on high-field small- animal scanners. Similar to clinical measurement, this method provides a complete spatially and temporally resolved dataset of the murine cardiovascular blood flow and allows for threedimensional flow pattern analysis.