Oxidation of aliphatic, branched chain, and aromatic hydrocarbons by Nocardia cyriacigeorgica isolated from oil-polluted sand samples collected in the Saudi Arabian Desert
Oxidation of aliphatic, branched chain, and aromatic hydrocarbons by Nocardia cyriacigeorgica isolated from oil-polluted sand samples collected in the Saudi Arabian Desert
复制标题
DOI：
10.1002/jobm.200900358
复制
发表时间：
2010-06-01
影响因子：
3.1
通讯作者：
Schauer, Frieder
中科院分区：
文献类型：
--
作者：
Nhi-Cong, Le Thi;Mikolasch, Annett;Schauer, Frieder
从沙特阿拉伯沙漠石油污染沙样中分离到的一株土壤细菌被鉴定为环状诺卡氏菌，它具有很高的降解和利用广泛碳氢化合物的能力。通过GC/MS和HPLC/UV-Vis分析无细胞提取物中的代谢物，并与标准化合物进行比较，阐明了三类碳氢化合物的代谢途径。在十四烷氧化过程中，有十四醇、十四酸、十二酸、十一酸作为代谢物，表明正构烷烃的单端降解途径。Pristane的氧化导致了伯斯坦酸、2-甲基戊二酸、4，8-二甲基壬酸和2，6-二甲基庚酸的存在，这可能导致单端和双端的氧化。对于仲辛基苯，检测到8种代谢物，包括5-苯基己酸、3-苯基丁酸、2-苯基丙酸、β-甲基肉桂酸、苯乙酮、β-羟基苯乙酮、2，3-二羟基苯甲酸和琥珀酸。从这些中间体出发，研究了仲辛基苯的一种新的降解途径。结果表明，该菌能有效降解脂肪烃、支链烷烃和烷基苯，是一种适合于石油或石油产品生物降解的细菌。
A soil bacterium isolated from oil-polluted sand samples collected in the Saudi Arabian Desert has been determined as Nocardia cyriacigeorgica, which has a high capacity of degrading and utilizing a broad range of hydrocarbons. The metabolic pathways of three classes of hydrocarbons were elucidated by identifying metabolites in cell-free extracts analyzed by GC/MS and HPLC/UV-Vis in comparison with standard compounds. During tetradecane oxidation, tetra-decanol; tetradecanoic acid; dodecanoic acid; decanoic acid could be found as metabolites, indicating a monoterminal degradation pathway of n-alkanes. The oxidation of pristane resulted in the presence of pristanoic acid; 2-methylglutaric acid; 4,8-dimethylnonanoic acid; and 2,6-dimethylheptanoic acid, which give rise to a possible mono- and di-terminal oxidation. In case of sec-octylbenzene, eight metabolites were detected including 5-phenylhexanoic acid; 3-phenylbutyric acid; 2-phenylpropionic acid; beta-methylcinnamic acid; acetophenone; beta-hydroxy acetophenone; 2,3-dihydroxy benzoic acid and succinic acid. From these intermediates a new degradation pathway for sec-octylbenzene was investigated. Our results indicate that N. cyriacigeorgica has the ability to degrade aliphatic and branched chain alkanes as well as alkylbenzene effectively and, therefore, N. cyriacigeorgica is probably a suitable bacterium for biodegradation of oil or petroleum products in contaminated soils.