Tuning the specificity of a synthetic receptor using a selected nucleic acid receptor
Tuning the specificity of a synthetic receptor using a selected nucleic acid receptor
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DOI：
10.1021/ja0478476
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发表时间：
2004-12-22
影响因子：
15
通讯作者：
Anslyn, EV
中科院分区：
文献类型：
--
作者：
Manimala, JC;Wiskur, SL;Anslyn, EV
由于其相对简单，合成受体通常缺乏生物聚合物受体（如适体）所观察到的选择性。然而，配体的适体识别受到四种典型核苷酸固有的化学性质的限制。在这里，我们报道了三元配合物的设计和选择，其中双硼酸合成宿主(1)与各种羧酸结合的特异性由周围的适体调节。虽然单独合成受体对柠檬酸盐的选择性高于dl -酒石酸盐，但适体受体复合物的形成逆转了有机宿主的选择性，优先结合酒石酸盐。在引入合成宿主后，RNA构象发生了变化，与诱导配合机制相一致。
Because of their relative simplicity, synthetic receptors often lack the selectivity observed for biopolymer receptors, such as aptamers. However, aptamer recognition of ligands is limited by the chemistries inherent in the four canonical nucleotides. Here, we report the design and selection of a ternary complex in which the specificity of a bis-boronic acid synthetic host (1) that binds to various carboxylic acids is tuned by a surrounding aptamer. Although, the synthetic receptor alone has higher selectivity for citrate over DL-tartrate, the formation of the aptamer: receptor complex reversed the organic host selectivity to preferentially bind tartrate. The RNA conformation changed upon the introduction of the synthetic host, consistent with an induced-fit mechanism for binding.